Highly Efficient Beads for Bacterial Cell Disruption

Scripps Research California
Postdoctoral Associate

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Company:

BioSpec Products

Product Name:

0.1 mm Diameter Glass Beads

Catalog Number:

11079101

We used BioSpec 0.1 mm glass beads for mechanical lysis of Enterococcus faecium cells using the FastPrep-24™ 5G Homogenizer to extract proteins for downstream Western blot analysis. We chose this product due to its optimal bead size for efficient disruption of Gram-positive bacterial cell walls and its compatibility with high-speed homogenizers. The goal was to obtain high-quality protein lysates for studying cell wall hydrolase expression. I would recommend this product for bacterial lysis applications. Pros include effective and reproducible lysis and ease of use; the main con is that bead reuse is limited in sterile workflows.

Application :

Used for mechanical disruption of Gram-positive bacterial cells to extract proteins and nucleic acids for molecular analysis.

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