Antibody drug conjugates (ADCs) are a fast-growing class of biomolecule. The ratio of the conjugated drug to mAb (drug-to-antibody ratio or DAR) is a critical quality attributes for ADC efficacy and safety. Monitoring the changes in DAR distribution in vivo is crucial, but sample preparation steps for measurement of average DAR and DAR distribution in circulation by LC/MS can be tedious and error-prone. This Application Note demonstrates a streamlined ADC DAR workflow using the AssayMAP Bravo, with an Agilent 6545XT AdvanceBio LC/Q-TOF, and Agilent BioConfirm DAR Calculator software. The workflow is simple to implement, highly reproducible, scalable, and minimizes hands-on time.

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