Isolating nuclei is a critical step for single nuclear RNA-sequencing and ATAC-sequencing workflows. Ensuring that non-clustered, debris-free samples are obtained is crucial to successful library preparation. Dual fluorescence counting using the CellDrop™ FL Automated Cell Counter can clearly distinguish nuclei, unlysed intact cells, and debris by acridine orange/propidium iodide (AO/PI) counting. Learn more about counting nuclei from the mouse brain in this application note.

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