8 Aug 2013: Thermo Fisher Scientific has developed a simple and accurate method to quantify four key antibiotics in dried distillers grains with solubles (DDGS) using high-performance liquid chromatography with charged aerosol detection. Application Note (AN) 1055: Determination of Virginiamycin, Erythromycin, and Penicillin in Dried Distillers Grains with Solubles demonstrates the ability of a specialized protein and peptide column to efficiently separate and resolve the target antibiotics within 15 min, followed by column cleanup and equilibration for a total analysis time of 25 min.
The U.S. Food and Drug Administration has raised concern over food-producing animals consuming DDGS with antibiotic residues, which potentially may lead to increased antibiotic resistance in humans and animals. Therefore, to meet possible future regulatory requirements, a screening method is needed to determine residual antibiotics in DDGS. The multistep sample preparation process and chromatography detailed in AN 1055 provide a quantification method that addresses the challenge of determining the four major antibiotics, with a wide range of physical and chemical properties, used in DDGS production.
This application note and many others can be found at www.thermoscientific.com/dionex under the Documents tab.
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