Supercritical Fluid Chromatography (SFC) systems analyse and purify low to moderate molecular weight molecules and are particularly useful for their ability to separate chiral compounds. SFC is similar to HPLC in that it relies on differences in polarity between the column (or stationary phase) and the solvent (or mobile phase) for separation, but is unique in that CO2 is employed as the solvent in the mobile phase and thus must be pressurized. By maintaining a cold environment and particular pressure, the CO2 can reach a supercritical state. Detection can be done in a number of ways including UV/VIS, mass spectrometry, FID and evaporative light scattering. Factors that affect the process of SFC include the flow rate, choice of co-solvent to be used alongside CO2, column selectivity, and scalability.
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| Company | JASCO | JASCO | JASCO |
| Item | SFC-4000 Analytical Supercritical Fluid Chromatography (SFC) System | SFC-4000 Preparative Supercritical Fluid Chromatography (SFC) System | SFC-4000 SFC-FID (for Fuel Analysis by ASTM D5186 and D6550) |
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| Catalog Number | SFC-4000 | PR-4088 | SFC-4000 |
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| Description | The SFC-4000 Analytical SFC System provides flexible configurations for any type of separation. The SFC-4000 can be set-up for use as a single column/single detector system or as a multi-column/multi-detector system for rapid method development. The SFC-4000 has an option for up to three column The SFC-4000 Analytical SFC System provides flexible configurations for any type of separation. The SFC-4000 can be set-up for use as a single column/single detector system or as a multi-column/multi-detector system for rapid method development. The SFC-4000 has an option for up to three column ovens each with ten full sized columns; each with separate temperature control, great for combining chiral and achiral SFC in a single system. Take advantage of a PDA detector combined with the CD-4095 chiral detector to work up fast analytical methods for direct scale-up to semi-prep and prep separations of chiral compounds.ChromNAV is an easy to use data system with a user-friendly interface and comprehensive automated data analysis. The ChromNAV Method Scouting Module is used for fast column and solvent screening.... Read More | The preparative SFC system is used for large scale purification using columns with IDs of 10, 20, and 30 mm. The system can be used for multiple sample library purification or individual component large-scale purification, with sample injection either using a preparative autosampler or syringe basedThe preparative SFC system is used for large scale purification using columns with IDs of 10, 20, and 30 mm. The system can be used for multiple sample library purification or individual component large-scale purification, with sample injection either using a preparative autosampler or syringe based large volume injector. Fraction collection can be triggered based on time, threshold or slope from up to 4 different detector signals including PDA, UV, CD, and/or MS. The flexible fraction layout includes open-bed fraction collection for a larger number of fractions for multiple samples or valve based with up to 8 fractions for repeated injections producing large fraction volumes. ChromNAV provides complete integrated control for flow rate, temperature, sample injection and fractionation. The SFC-4000 Preparative systems can be used for purifications using 10mm, 20mm or 30mm ID columns. The flexible configuration can be easily changed to suit a basic single column/single detector system or a multi-column/multi-detector system. Fraction collection options range from simple time-based 6 or 8 fractions to sophisticated threshold based open-bed fraction collection for up to 80 fractions. System control provides simple acquisition of chromatograms, automated data analysis, manual and automated fraction collection and a method scouting module for fast solvent and column screening.... Read More | The fully automated SFC-FID System provides fast and accurate analysis using JASCO’s unique high performance SFC columns – SFCpak SIL PA and SFCpak-SIL PAAGS. The SFC-FID system comprises an analytical CO2 pump with autosampler, column oven, and back pressure regulator, with FID (and splitter oven).The fully automated SFC-FID System provides fast and accurate analysis using JASCO’s unique high performance SFC columns – SFCpak SIL PA and SFCpak-SIL PAAGS. The SFC-FID system comprises an analytical CO2 pump with autosampler, column oven, and back pressure regulator, with FID (and splitter oven). Optional UV or PDA detection is available for simultaneous measurement of samples using UV absorption and FID. The SFC-FID System has been developed as a simple and validated ‘turn-key’ solution for the measurement of the two ASTM standard measurements that require SFC. ASTM-5186 for aromatics analysis, in diesel and aviation fuel and D-6550 for olefin content in gasoline. The SFC-FID system can also be configured for any analysis that may require SFC for the separation of volatile materials such as oils, fuels and lubricants.... Read More |
| Type | Analytical Supercritical Fluid Chromatography (SFC) System | Preparative Supercritical Fluid Chromatography (SFC) System | Supercritical Fluid Chromatography (SFC) Flame Ionization Detection (FID) System |
| Detector(s) | Standard: PDA / Options: UV-Visible, Chiral (Circular Dichroism), Mass Spectrometer | Standard: PDA / Options: UV-Visible, Chiral (Circular Dichroism), Mass Spectrometer | Flame Ionization Detector |
| Flow Rate | PU-4180: 0.5 to 10 mL/min / PU-4380: 0.5 to 8 mL/min | PU-4088: 10 to 150 mL/min / PU-4388: 10 to 150 mL/min | PU-4380: up to 10 mL/min (conventional to semi-prep, up to 10 mm I.D. column) |
| Max. Pressure | 500 Bar (50 MPa) | 500 Bar (50 Mpa) | 500 Bar (50 Mpa) |
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