Supercritical Fluid Chromatography (SFC) systems analyse and purify low to moderate molecular weight molecules and are particularly useful for their ability to separate chiral compounds. SFC is similar to HPLC in that it relies on differences in polarity between the column (or stationary phase) and the solvent (or mobile phase) for separation, but is unique in that CO2 is employed as the solvent in the mobile phase and thus must be pressurized. By maintaining a cold environment and particular pressure, the CO2 can reach a supercritical state. Detection can be done in a number of ways including UV/VIS, mass spectrometry, FID and evaporative light scattering. Factors that affect the process of SFC include the flow rate, choice of co-solvent to be used alongside CO2, column selectivity, and scalability.
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| Company | Shimadzu | Shimadzu | Shimadzu |
| Item | Nexera UC On-line SFE-SFC System | Nexera Preparative Supercritical Fluid Chromatography System | Nexera UCs UHPLC/SFC Switcing System |
| Citations | | | |
| Catalog Number | Nexera UC | Nexera Prep SFC | Nexera UC Switching |
| Price | | | |
| Description | The class-leading Nexera UC analytical SFC is perfect for scale up to prep SFC. It can be configured to automatically select 12 columns, form gradients with blends of up to 4 modifiers, and perform equilibration when exchanging columns and solvents. By automating these aspects of method development,The class-leading Nexera UC analytical SFC is perfect for scale up to prep SFC. It can be configured to automatically select 12 columns, form gradients with blends of up to 4 modifiers, and perform equilibration when exchanging columns and solvents. By automating these aspects of method development, it minimizes human error and maximizes throughput.
Key benefits:
- Very fast separation speed due to the relatively low viscosity of supercritical CO2
- Improved peak capacity and chromatographic resolution
- Efficient separation of analogues and/or chiral compounds by high penetration mobile phase
- Different separation mode leads to high sensitivity
- Ideal for simultaneous analysis of compounds with a wide range of polarities
- Small-volume fractionation is possible
- Multiple configurations available, including SFE-SFC-MS system, SFC-MS/MS system, Chiral Screening System, and SFC-UV system
- Extensive column lineup
With dedicated Method Scouting Solution software, the columns and modifiers to be used can be managed in a database, and various analytical conditions can be applied by simply selecting them
from the graphical user interface (GUI). It supports evaluation of analytical conditions in multiple ways: calculating modifier and sample amounts required, prevention of degradation through column rinsing and changeover of enclosed fluids post-analysis, and prediction of analysis completion times.... Read More | Developed in collaboration with the Enabling Technologies Consortium, the award-winning Nexera UC Prep SFC is a next-generation solution to the demand for efficient and robust semi-prep SFC purification in the pharmaceutical, chemical and food industries. Not only does the Nexera Prep SFC achieve Developed in collaboration with the Enabling Technologies Consortium, the award-winning Nexera UC Prep SFC is a next-generation solution to the demand for efficient and robust semi-prep SFC purification in the pharmaceutical, chemical and food industries. Not only does the Nexera Prep SFC achieve superior fractionation recovery rates for preparative purification, it provides flexible system configurations in a compact design, minimizing bench footprint and allowing you to maximize lab resources.
- Patented LotusStream GLS technology achieves higher recovery rates with minimized carry-over, and allows for easy rinsing compared to earlier cyclone-style or centrifugal type GLS systems.
- Faster separation, lower solvent consumption
- Stacked injection function allows samples to be injected continuously without any waiting time, enabling more samples to be processed
- Compact, streamlined design includes a carbon dioxide pump that does not require an external chiller. In addition, one unit can handle a wide range of flow rates, lowering installation costs.
- Dedicated software with intuitive preparative settings ensures peaks can be separated reliably by users of all experience levels
- Extensive column lineup for analytical and preparative scale
... Read More | This system can switch automatically between SFC analysis and UHPLC analysis and make measurements on a single sample in each separation mode. It enhances user-friendliness and efficiency by allowing the investigation of separation conditions and performing reverse-phase high-speed analysis in a This system can switch automatically between SFC analysis and UHPLC analysis and make measurements on a single sample in each separation mode. It enhances user-friendliness and efficiency by allowing the investigation of separation conditions and performing reverse-phase high-speed analysis in a single system.
Key benefits:
- Enables both UHPLC and SFC analysis using a single system
- Reduces laboratory space requirements and equipment costs
- Existing UHPLC can be upgraded to an SFC/LC Switching System
- Method Scouting Solution software automates and manages method development analyses; switching from UHPLC to SFC conditions is performed without user intervention
- Reliable analysis even when switching between SFC and UHPLC modes
... Read More |
| Type | Supercritical Fluid Chromatography | Inquire | Inquire |
| Detector(s) | UV/VIS; PDA; LCMS | Inquire | Inquire |
| Flow Rate | 0.0001 to 5 mL/min | Inquire | Inquire |
| Max. Pressure | 66 MPa | Inquire | Inquire |
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