An HPLC detector is used to analyze the components of the eluted mixture that is collected after being run through an HPLC column. Differences in detector type are based on what is being detected. Detection can be done by measuring absorbance, changes in reflex indices, molecular weight, and fluorescence among others. When selecting a detector, it is important to consider the sample type being analyzed. Some sample types will not work or will have lower sensitivity on particular detectors. Also of consideration is the type of information that is being sought by the analysis and what method will be used when running the column, for example, some detectors will work with the gradient method while others will not.
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| Company | Waters | Wyatt Technology | Waters | Wyatt Technology |
| Item | Optilab™ Refractive Index Detector for HPLC/GPC | microOptilab™ Refractive Index Detector for UHPLC |
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| Catalog Number | Optilab | microOptilab |
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| Type | Refractive Index Detector | Refractive Index Detector |
| Detector(s) | Differential Refractive Index (dRI) | Differential Refractive Index (dRI) |
| Applications | GPC/SEC analysis of macromolecules by SEC-MALS | Measure concentrations of all types of samples in almost all solvents |
| Description | The Optilab™ is a unique dRI detector for standard HPLC/GPC with 256 times the detection power and 50 times the dynamic range over alternative RI detectors for chromatography. This detector measures the solvent's absolute refractive index, a requirement for MALS calculations, at the same The Optilab™ is a unique dRI detector for standard HPLC/GPC with 256 times the detection power and 50 times the dynamic range over alternative RI detectors for chromatography. This detector measures the solvent's absolute refractive index, a requirement for MALS calculations, at the same wavelength as the MALS detector, for maximum accuracy. With an operating window from 4°C - 65°C it is appropriate for both protein and polymer samples. Key benefits include its extraordinary range: -0.0047 to +0.0047 RIU (comparable to 25 mg/mL protein in PBS); the high-concentration version extends the range by 7x. Its sensitivity is ± 7.5 x 10-10 RIU across the entire measurement range. It also includes a high-resolution, touch-screen front panel that displays data graphs and diagnostics.... Read More | The microOptilab™ brings universal detection capabilities, unsurpassed concentration range, and superb sensitivity and linearity to the domain of UHPLC. Key benefits include low band broadening, < 4 µL, to maintain the resolution of very narrow UHPLC peaks. Its dRI measurement range is, - 4The microOptilab™ brings universal detection capabilities, unsurpassed concentration range, and superb sensitivity and linearity to the domain of UHPLC. Key benefits include low band broadening, < 4 µL, to maintain the resolution of very narrow UHPLC peaks. Its dRI measurement range is, - 4.7 x 10-3 to + 4.7 x 10-3 RIU (comparable to 25 mg/mL protein in PBS). The detector is highly sensitive, ± 1.5 x 10-9 RIU, across the entire measurement range. This detector measures solvent refractive index, required for MALS calculations at the same wavelength as the MALS detector, for maximum accuracy. The temperature range of 4°C - 65°C is appropriate for both proteins and polymers that require a range of temperatures. It also features a high-resolution, touch-screen front panel displays data graphs, system health indicators and instrument diagnostics.... Read More |
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