An HPLC detector is used to analyze the components of the eluted mixture that is collected after being run through an HPLC column. Differences in detector type are based on what is being detected. Detection can be done by measuring absorbance, changes in reflex indices, molecular weight, and fluorescence among others. When selecting a detector, it is important to consider the sample type being analyzed. Some sample types will not work or will have lower sensitivity on particular detectors. Also of consideration is the type of information that is being sought by the analysis and what method will be used when running the column, for example, some detectors will work with the gradient method while others will not.
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Company | Waters | Wyatt Technology | Waters | Wyatt Technology |
Item | microOptilab™ Refractive Index Detector for UHPLC | miniDAWN™ Multi-Angle Light Scattering (MALS) Detector for SEC-MALS |
Citations | | |
Catalog Number | microOptilab | miniDAWN |
Price | | |
Type | Refractive Index Detector | MALS and optional DLS |
Detector(s) | Differential Refractive Index (dRI) | High-gain, high-dynamic range photodiodes at 3 detection angles for MALS. Optional avalanche photodiode for simultaneous dynamic light scattering measurements. |
Applications | Measure concentrations of all types of samples in almost all solvents | With SEC/GPC: determination of distributions of absolute molar mass, size, conformation, conjugate ratio. With Calypso CG-MALS: characterization of biomolecular interactions. |
Description | The microOptilab™ brings universal detection capabilities, unsurpassed concentration range, and superb sensitivity and linearity to the domain of UHPLC. Key benefits include low band broadening, < 4 µL, to maintain the resolution of very narrow UHPLC peaks. Its dRI measurement range is, - 4The microOptilab™ brings universal detection capabilities, unsurpassed concentration range, and superb sensitivity and linearity to the domain of UHPLC. Key benefits include low band broadening, < 4 µL, to maintain the resolution of very narrow UHPLC peaks. Its dRI measurement range is, - 4.7 x 10-3 to + 4.7 x 10-3 RIU (comparable to 25 mg/mL protein in PBS). The detector is highly sensitive, ± 1.5 x 10-9 RIU, across the entire measurement range. This detector measures solvent refractive index, required for MALS calculations at the same wavelength as the MALS detector, for maximum accuracy. The temperature range of 4°C - 65°C is appropriate for both proteins and polymers that require a range of temperatures. It also features a high-resolution, touch-screen front panel displays data graphs, system health indicators and instrument diagnostics.... Read More | Couple the miniDAWN™ to your favorite HPLC or FPLC to determine the absolute molar masses and sizes of macromolecules and nanoparticles in solution from hundreds to millions of Daltons—without the need for column calibration standards. This sensitive unit requires as little as 25 ng injected Couple the miniDAWN™ to your favorite HPLC or FPLC to determine the absolute molar masses and sizes of macromolecules and nanoparticles in solution from hundreds to millions of Daltons—without the need for column calibration standards. This sensitive unit requires as little as 25 ng injected sample, 100 kDa polystyrene in THF (varies for other samples and solvents). The miniDAWN is engineered with self-contained modules that are easily replaced making it field serviceable. The system-ready monitors soise levels in real-time and an all-green status indicator lets you know when your system is ready for data collection. The optional DLS module uses the WyattQELS™ module, which fits inside the miniDAWN to providing on-line dynamic light scattering for determining radii down to 0.5 nm. All light scattering cells are subject to contamination by particles and to combat this issue the instrument has a self-cleaning, embedded COMET™ ultrasonic module. For maximum reliability and uptime, program the miniDAWN to automatically clean the cell after every run. The units may also be combined with a Calypso™ composition-gradient system to characterize biomolecular interactions.... Read More |
Wavelength Range | Inquire | Inquire |
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