An HPLC detector is used to analyze the components of the eluted mixture that is collected after being run through an HPLC column. Differences in detector type are based on what is being detected. Detection can be done by measuring absorbance, changes in reflex indices, molecular weight, and fluorescence among others. When selecting a detector, it is important to consider the sample type being analyzed. Some sample types will not work or will have lower sensitivity on particular detectors. Also of consideration is the type of information that is being sought by the analysis and what method will be used when running the column, for example, some detectors will work with the gradient method while others will not.
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| Company | JASCO | JASCO | JASCO | JASCO | JASCO |
| Item | LC-4000 Series Fluorescence Detectors | LC-4000 Circular Dichroism Detector | LC-4000 Series PDA Detectors | LC-4000 Series Refractive Index Detectors | LC-4000 Series UV/Visible Detectors |
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| Catalog Number | FP-4020 / FP-4025 | CD-4095 | MD-4010 / MD-4015 / MD-4017 | RI-4030 / RI-4035 | UV-4070 / UV-4075 / UV-4570 / UV-4575 |
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| Type | Fluorescence Detector | Circular Dichroism Detector | Diode Array Detector | Refractive Index Detector | UV-Visible Detector |
| Detector(s) | Fluorescence | Circular Dichroism | Diode Array | Refractive Index | UV-Visible |
| Description | The LC-4000 Series Fluorescence Detectors have been designed for the pursuit of extreme sensitivity. A complete redesign of the optics and electronics, as well as removing the effects temperature fluctuation with a new temperature regulated cell body has led to the highest sensitivity with stable The LC-4000 Series Fluorescence Detectors have been designed for the pursuit of extreme sensitivity. A complete redesign of the optics and electronics, as well as removing the effects temperature fluctuation with a new temperature regulated cell body has led to the highest sensitivity with stable detection.
The new axially irradiated cell design enables the very highest sensitivity detection. These changes have resulted in a leap in sensitivity with signal noise ratio of 2300:1 or higher (water Raman peak). The FP-4025 is an excellent routine fluorescence detector that has a water Raman S/N of 1400:1.
Both detectors can be used for simultaneous detection at two wavelength pairs; excitation and emission wavelengths can be changed during the acquisition using a time program and automatic spectra scanning can be triggered manually, by time or automatically by threshold. A built-in mercury lamp enables automatic wavelength correction and the front-access design allows easy replacement of the flow cell assembly and light source.... Read More | The CD-4095 HPLC Detector for measuring circular dichroism developed from the market leading J-Series circular dichroism spectropolarimeter. Optically active compounds with a chromophore close to a chiral center may absorb circularly polarized light, which can be detected with excellent sensitivity The CD-4095 HPLC Detector for measuring circular dichroism developed from the market leading J-Series circular dichroism spectropolarimeter. Optically active compounds with a chromophore close to a chiral center may absorb circularly polarized light, which can be detected with excellent sensitivity and selectivity. When chiral compounds are measured using a UV-Visible detector, the d- and l- enantiomers cannot be distinguished even when separated by a chiral column.
The CD-4095 circular dichroism detector makes it possible to positively distinguish between the d- and l- enantiomers by measuring the positive and negative peaks. It is also possible to quantify the d- and l-forms even when they are not separated, a calibration curve based on the g-factor can be used to determine the enantiomeric ratio. The ChromNAV CDS can be used to simultaneously measure the CD, g-factor data and UV-visible signal. The CD spectra of eluted compounds can be measured by triggering scanning manually, by timed program or automatically by threshold.... Read More | The LC-4000 Series includes three PDA detectors.
The MD-4010 PDA detector has high sensitivity, comparable to a single wavelength detector. It uses a 1024 element photometric diode array, with measurement wavelength range from 190 nm to 900 nm and resolution of 1 nm. Fast spectral acquisition rateThe LC-4000 Series includes three PDA detectors.
The MD-4010 PDA detector has high sensitivity, comparable to a single wavelength detector. It uses a 1024 element photometric diode array, with measurement wavelength range from 190 nm to 900 nm and resolution of 1 nm. Fast spectral acquisition rate of up to 100 spectra/sec. The temperature controlled SP type semi-micro flow cell is used narrow peaks typically found with fast UHPLC or RHPLC separations. An integrated mercury lamp is used for automatic wavelength calibration and validation.
The MD-4015 PDA detector is similar to the MD-4010 with equivalent sensitivity, covering the wavelength range from 200 nm to 650 nm using a 512 element PDA for 1nm resolution, and with high speed 100 Hz spectral acquisition.
The MD-4017 uses a CMOS detector covering the UV region from 200-400nm; with 1nm resolution and up to 20Hz spectrum acquisition; useful for peak widths as small as 1 – 2 seconds.... Read More | The LC-4000 Refractive Index Detectors include an optical system mounted in a precise temperature-regulated housing to eliminate the influence of changes in ambient temperature. Both detectors are fully stable just one hour after powering on. The LED light source has a long lifetime (over 10,000 The LC-4000 Refractive Index Detectors include an optical system mounted in a precise temperature-regulated housing to eliminate the influence of changes in ambient temperature. Both detectors are fully stable just one hour after powering on. The LED light source has a long lifetime (over 10,000 hours) for low maintenance. An optional pressure release safety-valve can be fitted to prevent damage to the flow cell in the event blockage.
The RI-4030 Detector can be used for analytical and preparative scales. For preparative separations, the maximum flow rate is 120 mL/min.
The RI-4035 is a dedicated semi-micro RI detector with a micro-volume flow cell designed for use with fast semi-micro GPC analysis using columns with a fine pore size. The 100Hz data acquisition rate makes the RI-4035 suitable for RHPLC and UHPLC separations.... Read More | The UV-4070 (190 to 900 nm) and UV-4075 (190 to 600 nm) UV-Visible HPLC Detector uses an advanced Czerny-Turner monochromator with dual wavelength data acquisition (up to 100 Hz for UHPLC) and spectral scanning functions. Baseline change due to refractive index is minimized by the flow cell design, The UV-4070 (190 to 900 nm) and UV-4075 (190 to 600 nm) UV-Visible HPLC Detector uses an advanced Czerny-Turner monochromator with dual wavelength data acquisition (up to 100 Hz for UHPLC) and spectral scanning functions. Baseline change due to refractive index is minimized by the flow cell design, and fluctuation from changes in environmental and lamp temperature are reduced with a regulated lamp housing and temperature controlled cell.
The UV-4570 Compact UV-Visible HPLC Detector is only 6 inches wide (15 cm) and can be used stand-alone or as part of a system for single wavelength measurement from the UV to Visible region (190 to 900 nm). Cassette-type tapered flow-cells and user replaceable lamps. Programmable wavelength switching and spectral scanning. The UV-4575 UV-Visible HPLC Detector... Read More |
| Wavelength Range | 200 to 700 nm (Option up to 900 nm) | 220 to 460 nm | MD-4010: 190 to 900 nm / MD-4015: 200 to 600 nm / MD-4017: 200 to 400 nm | Inquire | UV 4070: 190 to 900 nm / UV 4075: 190 to 600 nm / UV-4570: 190 to 900 nm / UV-4575: 190 to 600 nm |
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