Chromatography columns are devices designed to separate chemical compounds using a stationary and a mobile phase. These columns, used in many types of both gas and liquid chromatography, vary greatly and are often highly specialized for the specific target compound. The Labcompare chromatography column search tool is designed to help you quickly find and compare columns from various leading manufacturers in order to enable you to quickly purchase the column that is right for your research.
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| Company | Shimadzu | Shimadzu |
| Item | Shim-pack Bio Diol – Size Exclusion Columns | Shim-pack Arata LC Columns |
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| Catalog Number | Bio Diol | Arata Columns |
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| Applications | Peptides, oligonucleotides, and biopharmaceuticals | Basic Compounds & Peptides |
| Pore Size | 6, 12, 20, and 30 nm | 12 nm (120 Å) |
| Description | Available at 2, 3, and 5 um particle sizes and ranging in length from 150 to 500 mm, Shim-pack Bio Diol LC columns are effective for analysis of aggregates and fragments of mAb, oligonucleotides and carbohydrates. By reducing the particle size from 5µm to 2µm, the resolution between Available at 2, 3, and 5 um particle sizes and ranging in length from 150 to 500 mm, Shim-pack Bio Diol LC columns are effective for analysis of aggregates and fragments of mAb, oligonucleotides and carbohydrates. By reducing the particle size from 5µm to 2µm, the resolution between aggregates and monomers was greatly improved. Furthermore, by reducing the column length from 300mm to 150mm using a 2µm particle, 50% less run time was achieved, while maintaining resolution as compared to the original method that used a 5µm, 4.6 × 300mm column.... Read More | Unprecedented Resolution and Peak Shape for High pH Compounds
Even for LC columns that claim to be designed for basic compounds, adequate resolution often cannot be obtained due to problems such as leading of highly polar basic compounds, peak shape deterioration of acidic compounds, or long Unprecedented Resolution and Peak Shape for High pH Compounds
Even for LC columns that claim to be designed for basic compounds, adequate resolution often cannot be obtained due to problems such as leading of highly polar basic compounds, peak shape deterioration of acidic compounds, or long equilibration time required for low ionic strength acidic mobile phase. All of these issues have been solved with Shim-pack Arata that was specifically designed to give unmatched peak shape for high pH compounds.
Unmatched Peak Shape
Unmatched peak shape of basic compounds could be achieved while maintaining good peak shape for acidic compounds using the Shim-pack Arata LC column. Even when low ionic strength acidic mobile phase, such as 0.1% formic acid containing mobile phase are used, excellent peak shape of both amitriptyline (a basic compound) and benzoic acid (an acidic compound) could be achieved.
Excellent Separation Performance for Peptides Even with Weak Ion Pairing Acids
In order to obtain good peak shape of peptides under reversed phase chromatography, TFA containing mobile phases are frequently used which the ion pairing effect is relatively strong. However, TFA could cause ion suppression in LC/MS analysis. Excellent peak shape and separation performance for peptides can be achieved using the Shim-pack Arata LC column even with 0.1 % formic acid containing mobile phase.
Rapid Equilibration Even with Low Ionic Strength Acidic Mobile Phases
When analyzing basic compounds on a typical ODS column with low ionic strength acidic mobile phase, peak shape and long equilibration times are common problems. Shim-pack Arata LC columns can be rapidly equilibrated in low ionic strength acidic mobile phases yielding excellent peak shape and stable retention times.... Read More |
| Phase | Diol 60/120/200/300 | C18 |
| pH | 5.0 to 7.5 | 2 to 7.5 |
| Particle Size | 2, 3, and 5 µm | 2.2 µm, 5 µm |
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