| Description | Enzyme immunoassay for determination of Human BDNF concentrations in serum, plasma, cell culture supernatant and other biological samples. For research use only, not for use in diagnostic procedures. | Enzyme immunoassay (ELISA) for the determination of IgG class antibodies against Trypanosoma cruzi in human serum or plasma (citrate, heparin). For research use only, not for use in diagnostic procedures. | Indirect ELISA for the determination of human GPIHBP1 Autoantibody in serum, EDTA-plasma, heparin plasma, and post-heparin EDTA-plasma. For research use only, not for use in diagnostic procedures. | Solid phase direct sandwich ELISA for the determination of Lysozyme in human plasma, serum, or stool. FDA exempt, can be used for in-vitro diagnostics. | Tau protein is a 45-50kDa microtubule-associated protein which consists of 352-441 amino acids. It has a role of stabilizing microtubules that transport intracellular substances in nerve cells. It is known that tauopathy such as Alzheimer's type dementia (AD) and frontotemporal lobar degeneration (Tau protein is a 45-50kDa microtubule-associated protein which consists of 352-441 amino acids. It has a role of stabilizing microtubules that transport intracellular substances in nerve cells. It is known that tauopathy such as Alzheimer's type dementia (AD) and frontotemporal lobar degeneration (FTLD) is developed due to abnormal accumulation of Tau. Three features of AD, senile plaque, neurofibrillary tangle and neuronal cell death have been observed in the brain of AD subjects and it is considered that neurofibrillary tangle is triggered by abnormal accumulation of Tau. The main element of neurofibrillary tangle is called PHF (paired helical filament) and it is accumulated in nerve cells. Overexpressed phosphorylated tau is aggregated and it becomes a main cause of PHF. It has been thought that GSK-3ß is involved in the process of phosphorylation of tau and Aß promotes the activity for phosphorylation of tau. It has been observed that the concentration of Tau is very high in CSF of subjects with neurodegenerative diseases such as Alzheimer's disease as it is released in CSF due to neuron is destroyed while it is rarely detected in CSF of healthy subjects. This ELISA is for the determination of human phosphorylated Tau (181) in CSF. For research use only, not for use in diagnostic procedures.... Read More |
| Method | Enzyme-Linked Immunosorbent Assay (ELISA) | Enzyme-Linked Immunosorbent Assay (ELISA) | Enzyme-Linked Immunosorbent Assay (ELISA) | Enzyme-Linked Immunosorbent Assay (ELISA) | Enzyme-Linked Immunosorbent Assay (ELISA) |
| Assay Time | 90 min. incubation (37°C) + 60 min. (37°C) + 30 min. (37°C) + 20 min. (37°C) = 3 hours, 20 min. total incubation time. | 1 hr 45 mins (incubation) | 2 hour total incubation time. | 1 hr 15 mins (incubation) | Overnight + 1 hour (incubation) |