| Description | Product Introduction:This kit is used to enrich low-abundance proteins in plasma samples and rapidly prepare proteomic samples, and the processed samples can be used for subsequent mass spectrometry detection.Product Components and Storage Conditions:M1456374Component10T25TStorageM1456374 AMagnetic Product Introduction:This kit is used to enrich low-abundance proteins in plasma samples and rapidly prepare proteomic samples, and the processed samples can be used for subsequent mass spectrometry detection.Product Components and Storage Conditions:M1456374Component10T25TStorageM1456374 AMagnetic beads Max200 µL500 µL4℃M1456374 BIncubation Buffer I4.8 mL12 mLRTM1456374 CIncubation Buffer II6 mL15 mLRTM1456374 DWashing Buffer6 mL15 mLRTM1456374 ELysis Buffer0.6 mL1.5 mL-20℃. Store in the dark.M1456374 FBalance Buffer2.8mL7 mL4℃M1456374 GDigest Buffer16 µL40 µL-20℃M1456374 HStop Buffer300 µL750 µLRTM1456374 IWash Buffer I4 mL10 mLRTM1456374 JWash Buffer II2.4 mL6 mLRTM1456374 KWash Buffer III2.4 mL6 mLRTM1456374 LElution Buffer4.8 mL12 mLRT. Store in the dark.M1456374 MLoading Buffer120 µL300 µLRTM1456374 NTip pillar10T25TRTOperating Procedure: 1.Centrifuge the plasma sample (3000g, 10min), and take the supernatant for later use (if storage is required, store it at -80°C for long-term preservation to avoid repeated freezing and thawing).2.Take 50-100µL of centrifuged plasma, add 400µL of Incubation buffer I, then add 18µL of Magnetic beads, vortex to mix, and incubate at room temperature on a shaking mixer for 1 hour.3.After incubation, use a magnetic rack to magnetically separate for 3min, and discard the supernatant.4.Remove the EP tube, add 500µL of Incubation buffer II, gently invert up and down to mix several times, magnetically separate for 3min, and discard the supernatant.5.Remove the EP tube, add 500µL of Washing Buffer, gently invert up and down to mix several times, magnetically separate for 3min, and discard the supernatant.6.Remove the EP tube, add 50µL of Lysis Buffer to resuspend the beads, place in a water bath at 95°C for 10min, then take it out and cool to room temperature.7.Add 225µL of Balance buffer to the EP tube that has cooled to room temperature.8.Add 1µL of Digest buffer to the sample, and perform enzymatic digestion with shaking in a metal bath at 37°C and 1200rpm for 3-16h.9.After enzymatic digestion, take out the EP tube, add 25µL of Stop buffer to the sample, and vortex to mix.10.Add 320µL of Wash buffer I, shake vigorously for 3min, centrifuge at 15000rpm for 3min, and remove the upper liquid.11.Transfer the lower layer sample into the Tip column, centrifuge at 2500rpm for 3-5min until all the liquid is centrifuged down. If the liquid flow rate is slow, the rotation speed can be appropriately increased.12.Add 200µL of Wash buffer II (shake for 10-20s before use) to the desalting column, centrifuge at 2500rpm for 3-5min until all the liquid is centrifuged down.13.Add 200µL of Wash buffer III to the desalting column, centrifuge at 2500rpm for 3-5min until all the liquid is centrifuged down.14.Put the desalting column into a new EP tube, add 200µL of Elution buffer to the desalting column, centrifuge at 2000rpm for 3-5min until all the liquid is centrifuged down.15.Repeat the previous step, collect the eluates from both times, and freeze-dry them.16.Add 10µL of Loading buffer, vortex vigorously for 3min, centrifuge at 2000g for 10min, take an appropriate amount of sample for mass spectrometry detection. Taking the HF-X instrument as an example, 0.5-1µg of sample is sufficient for loading. Automated Operation Process:It is compatible with mass spectrometry proteomics pretreatment workstations, enabling one-stop processing from plasma to peptides. This eliminates manual operation errors, improves the reproducibility of sample preparation workflows, and provides high precision and reliability for various laboratory procedures... Read More | Inquire | DescriptionIt contains a set of six different heterogeneous palladium catalysts, useful for rapid screening of catalysis conditions. It is in sampler format with individual components packaged for multiple experiments and mini scale-up. The cost of the kit is less than the total cost of individual DescriptionIt contains a set of six different heterogeneous palladium catalysts, useful for rapid screening of catalysis conditions. It is in sampler format with individual components packaged for multiple experiments and mini scale-up. The cost of the kit is less than the total cost of individual components.Catalysis Screening Kits... Read More | DescriptionTruQuant IQQ is a high-quality quantitation system for making simultaneous accurate biological measurements on several hundred biochemicals in small quantities of biological samples. This is achieved by (1) spiking a complex Internal Standard (WORKFLOW-A) into a biological sample to a) DescriptionTruQuant IQQ is a high-quality quantitation system for making simultaneous accurate biological measurements on several hundred biochemicals in small quantities of biological samples. This is achieved by (1) spiking a complex Internal Standard (WORKFLOW-A) into a biological sample to a) quantify all the biochemicals in the sample relative to their counterparts in the Internal Standard, b) suppression-correct each compound and c) normalize sample to sample variances; and (2) injecting the same well characterized Long-Term Reference Standard (WORKFLOW-B) to create a daily retention time (RT) library of all compounds to be found in the Internal Standard for reproducible ID, and to measure day-to-day (QA/QC) to assure reproducible instrument performance. The system is completely automated using IROA ClusterFinder™software.IROA TruQuant IQQ Workflow Kit contains the materials and tools for the analysis of 90 experimental samples. The kit is intended to be used for mass spectrometry metabolomics applications... Read More | Products contentN665993Component240 TStorageN665993AIndex N501 Primers for Illumina240 µL-20℃. Avoid freeze/ Thaw cycle.N665993BIndex N925-N948 Primers for Illumina24×10 µL-20℃. Avoid freeze/ Thaw cycle. Products Introduction This kit is a companion kit to the transposase-Products contentN665993Component240 TStorageN665993AIndex N501 Primers for Illumina240 µL-20℃. Avoid freeze/ Thaw cycle.N665993BIndex N925-N948 Primers for Illumina24×10 µL-20℃. Avoid freeze/ Thaw cycle. Products Introduction This kit is a companion kit to the transposase-based Rapid DNA Library Construction Kit for Illumina platform library construction. Each kit contains one N5 primer and 24 N7 primers, which can be used to prepare 24 different single-ended Index libraries. All reagents provided in the kits have been subjected to stringent quality control and functional validation to maximize the stability and reproducibility of library construction. The libraries can be used for sequencing on Illumina platforms such as HiSeq X-10/4000/2500/2000 and MiSeq. Provide your own instruments, reagents and consumables1. Magnetic frame: DynaMagTM-2 is recommended.2. DNA purification and recovery kit: It is recommended to use Kangwei DNA purification and recovery kit by magnetic bead method.3. DNA building kit: It is recommended to use the Kangwei Century transposase method second-generation sequencing rapid DNA building kit.4. Anhydrous ethanol.5. Reaction tubes: It is recommended to use low adsorption PCR tubes with 1.5 ml centrifuge tubes;Tip: It is recommended to use a high quality filter tip to prevent contamination of kits and library samples. Pre-experiment Preparation and Important NotesPlease centrifuge briefly before opening the cap so that the liquid collects at the bottom of the tube to avoid cross-contamination between different primers. ProcedureFor the use of the CombiVision Second Generation Sequencing Multisample Primer Kit, please follow the CombiVision Second Generation Sequencing Rapid DNA Library Kit protocol.Index N501 Primer for IlluminaIndex N901-N996 Primer for Illumina... Read More |