Assay kits are used to carry out analyses in a broad scope of applications including the study of targets such as drugs, molecules, disease pathways, chemical elements or compounds, and organisms. They are a routine procedure in the modern pharmaceutical, medical and forensic world as well as research and university laboratories around the globe. Assay kits come with all the necessary reagents, instructions and protocols for fast and efficient processing, allowing for repeatable results.
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| Company | Aladdin Scientific Corporation | Aladdin Scientific Corporation | Aladdin Scientific Corporation | Aladdin Scientific Corporation | Aladdin Scientific Corporation |
| Item | ECL Pico Light Chemiluminescence Kit | DAB staining kit | Lipid Peroxidation (MDA) Assay Kit | Luxol Fast Blue Myelin Staining Kit | Victoria Blue Elastic Fiber Staining Kit |
| Catalog Number | E1491297 | D405772 | L486287 | L774828 | V774850 |
| Price | Supplier Page | Supplier Page | Supplier Page | Supplier Page | Supplier Page |
| Description | ECL Basic Chemiluminescence Detection Kit (Pico-grade) is a non-radioactive chemiluminescence system designed for detecting the activity of horseradish peroxidase (HRP) conjugated to membrane-bound proteins. This product contains an enhanced luminol substrate and a stable peroxide solution, enablingECL Basic Chemiluminescence Detection Kit (Pico-grade) is a non-radioactive chemiluminescence system designed for detecting the activity of horseradish peroxidase (HRP) conjugated to membrane-bound proteins. This product contains an enhanced luminol substrate and a stable peroxide solution, enabling the detection of trace amounts of protein as low as the pico-gram level. It is suitable for routine Western Blot chemiluminescence detection.Component ListComponent100 mL500 mLStorageE1491297AECL PicoLight Substrate50 mL250 mL2-8℃. Store in the dark.E1491297BECL PicoLight Oxidant50 mL250 mL2-8℃. Store in the dark.FeaturesHigh Sensitivity – Capable of rapidly detecting proteins at pico-gram levels, outperforming other high-end products in the same category.High Cost-Effectiveness – Offers exceptional value compared to other chemiluminescence substrates of similar grade.Instructions for UseRemove the transfer membrane from the washing buffer and drain any excess liquid, keeping the membrane moist.Mix PicoLight ECL Substrate and Oxidant in equal volumes to prepare the chemiluminescence working solution (approximately 0.1 mL per square centimeter of membrane).Apply the working solution evenly onto the membrane and incubate for 1–2 minutes. For strong target protein signals, avoid letting the working solution remain static. Gently agitate the membrane on a shaker for 1–2 minutes to prevent local depletion of reactants, which may affect the linear detection range.Remove the membrane and drain any excess working solution. Wrap it in a transparent protective cover.X-ray Film Exposure: In a darkroom, expose the membrane to X-ray film. Exposure times may vary from seconds to minutes. Develop the film and adjust exposure time for repeat imaging if necessary.CCD Imager: The membrane can be directly imaged without a protective cover. Follow the manufacturer’s instructions for image capture.The membrane can be stripped and reprobed for multiple hybridizations.PrecautionsProtect PicoLight ECL Substrate and the working solution from strong light to avoid reduced sensitivity.Due to the high sensitivity of the solution, avoid using overly concentrated antibodies to prevent high background signals.Sodium azide (NaN₃) inhibits HRP activity. Do not use NaN₃ for storing or reusing secondary antibodies.Wear a lab coat and disposable gloves for personal safety.This product is for research use only... Read More | Inquire | Lipid peroxidation is the degradation of lipids that occurs as a result of oxidative damage and is a useful marker for oxidative stress. Polyunsaturated lipids are susceptible to an oxidative attack, typically by reactive oxygen species, resulting in a well-defined chain reaction with the productionLipid peroxidation is the degradation of lipids that occurs as a result of oxidative damage and is a useful marker for oxidative stress. Polyunsaturated lipids are susceptible to an oxidative attack, typically by reactive oxygen species, resulting in a well-defined chain reaction with the production of end products such as malondialdehyde (MDA). Lipid peroxidation may contribute to the pathology of many diseases including atherosclerosis, diabetes, and Alzheimer′s.Lipid peroxidation (MDA) assay kit has been used to determine the levels of malondialdehyde (MDA).Suitability: Suitable for the measurement of malondialdehyde (MDA) in a variety of samples including tissue, cells and plasmaPrinciple: In this kit, lipid peroxidation is determined by the reaction of MDA with thiobarbituric acid (TBA) to form a colorimetric (532 nm)/fluorometric (λex= 532/λem= 553 nm) product, proportional to the MDA present... Read More | Inquire | Inquire |
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