Assay kits are used to carry out analyses in a broad scope of applications including the study of targets such as drugs, molecules, disease pathways, chemical elements or compounds, and organisms. They are a routine procedure in the modern pharmaceutical, medical and forensic world as well as research and university laboratories around the globe. Assay kits come with all the necessary reagents, instructions and protocols for fast and efficient processing, allowing for repeatable results.
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| Company | Molecular Devices, LLC | Molecular Devices, LLC |
| Item | EarlyTox™ Live/Dead Assay Kit | EarlyTox™ Live Cell Assay Kit |
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| Catalog Number | R8340 / R8341 | R8342 / R8343 |
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| Description | This kit enables the detection of both the live and dead populations of mammalian cells based on the integrity of the cell membrane using a fluorescence microplate reader. Calcein AM is a widely used live-cell marker. The non-fluorescent calcein AM permeates the intact cell membrane and is converted This kit enables the detection of both the live and dead populations of mammalian cells based on the integrity of the cell membrane using a fluorescence microplate reader. Calcein AM is a widely used live-cell marker. The non-fluorescent calcein AM permeates the intact cell membrane and is converted into the fluorescent calcein by intracellular esterases. The number of live cells is therefore indicated by the intensity of green fluorescence in the cytosol, with excitation at 495 nm and emission at 530 nm. EthD-III is virtually non-fluorescent and impermeant to an intact plasma membrane. In the event of compromised cell membrane integrity that is associated with cell death, EthD-III enters cells and binds to nucleic acids, resulting in bright red fluorescence in dead cells, with excitation at 530 nm and emission at 645 nm. Explorer Kit contains 1 vial of Calcein AM (4 mM, 30 µL) and 1 vial of EthD-III (2 mM, 60 µL). Each kit has enough material for two 96-well or 384-well microplates.
Bulk Kit contains 3 vials of Calcein AM (4 mM, 50 µL) and 2 vials of EthD-III (2 mM, 150 µL). Each kit has enough material for ten 96-well or 384-well microplates.... Read More | This kit enables the detection of the live population of mammalian cells using a fluorescence microplate reader based on the integrity of the cell membrane and the intracellular esterase activity. Calcein AM is a widely used live-cell marker. The non-fluorescent calcein AM permeates the intact cell This kit enables the detection of the live population of mammalian cells using a fluorescence microplate reader based on the integrity of the cell membrane and the intracellular esterase activity. Calcein AM is a widely used live-cell marker. The non-fluorescent calcein AM permeates the intact cell membrane and is converted into calcein, the fluorescent form, by intracellular esterases. The number of live cells is therefore indicated by the intensity of green fluorescence in the cytosol, with excitation at 495 nm and emission at 530 nm. The low cellular toxicity of calcein AM also enables long-term cell tracing, and cell proliferation assay. Explorer Kit contains 1 vial of Calcein AM (4 mM, 30 µL). Each kit contains enough material for two 96-well or 384-well microplates.
Bulk Kit contains 3 vials of Calcein AM (4 mM, 50 µL). Each kit contains enough material for ten 96-well or 384-well microplates.... Read More |
| Detection Target | Live and dead cells | Live cells |
| Method | Fluorescence Assay | Fluorescence Assay |
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