Assay kits are used to carry out analyses in a broad scope of applications including the study of targets such as drugs, molecules, disease pathways, chemical elements or compounds, and organisms. They are a routine procedure in the modern pharmaceutical, medical and forensic world as well as research and university laboratories around the globe. Assay kits come with all the necessary reagents, instructions and protocols for fast and efficient processing, allowing for repeatable results.
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| Company | Molecular Devices, LLC | Molecular Devices, LLC |
| Item | EarlyTox™ Caspase-3/7-D NucView 488 Assay Kit | EarlyTox™ Live Cell Assay Kit |
| Catalog Number | R8348 / R8349 | R8342 / R8343 |
| Price | | |
| Description | This kit enables enable detection of apoptosis in intact cell populations based on the measurement of caspase-3/7 activity using either a fluorescence microplate reader or a fluorescence imaging system. Caspase-3 and caspase-7 are proteases that are activated during the execution phase of apoptosis.This kit enables enable detection of apoptosis in intact cell populations based on the measurement of caspase-3/7 activity using either a fluorescence microplate reader or a fluorescence imaging system. Caspase-3 and caspase-7 are proteases that are activated during the execution phase of apoptosis. NucView 488 Caspase-3 substrate (formulated in DMSO or phosphate-buffered saline (PBS), for EarlyTox Caspase-3/7-D NucView 488 Assay Kit and EarlyTox Caspase-3/7 NucView 488 Assay Kit, respectively) is used to detect caspase-3/7 activity within intact cells without interfering with caspase activity. The substrate consists of a fluorogenic DNA dye that is coupled to the caspase-3/7 DEVD recognition sequence. Initially non-fluorescent, the substrate permeates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate, releasing a high-affinity DNA dye that migrates to the cell nucleus and stains DNA with bright green fluorescence, with excitation at 500 nm and emission at 530 nm. NucView 488 staining is formaldehyde-fixable and compatible with subsequent immunostaining. The assay kit includes an optional masking reagent to reduce background fluorescence when necessary. The PBS formulation is recommended for cells that are sensitive to DMSO.... Read More | This kit enables the detection of the live population of mammalian cells using a fluorescence microplate reader based on the integrity of the cell membrane and the intracellular esterase activity. Calcein AM is a widely used live-cell marker. The non-fluorescent calcein AM permeates the intact cell This kit enables the detection of the live population of mammalian cells using a fluorescence microplate reader based on the integrity of the cell membrane and the intracellular esterase activity. Calcein AM is a widely used live-cell marker. The non-fluorescent calcein AM permeates the intact cell membrane and is converted into calcein, the fluorescent form, by intracellular esterases. The number of live cells is therefore indicated by the intensity of green fluorescence in the cytosol, with excitation at 495 nm and emission at 530 nm. The low cellular toxicity of calcein AM also enables long-term cell tracing, and cell proliferation assay. Explorer Kit contains 1 vial of Calcein AM (4 mM, 30 µL). Each kit contains enough material for two 96-well or 384-well microplates.
Bulk Kit contains 3 vials of Calcein AM (4 mM, 50 µL). Each kit contains enough material for ten 96-well or 384-well microplates.... Read More |
| Detection Target | Caspase 3, 7 | Live cells |
| Method | Fluorescence Assay | Fluorescence Assay |
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