Assay kits are used to carry out analyses in a broad scope of applications including the study of targets such as drugs, molecules, disease pathways, chemical elements or compounds, and organisms. They are a routine procedure in the modern pharmaceutical, medical and forensic world as well as research and university laboratories around the globe. Assay kits come with all the necessary reagents, instructions and protocols for fast and efficient processing, allowing for repeatable results.
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| Company | Molecular Devices, LLC | Molecular Devices, LLC |
| Item | EarlyTox™ Caspase-3/7 NucView 488 Assay Kit | EarlyTox™ Caspase-3/7 R110 Assay Kit |
| Catalog Number | R8350 / R8351 | R8346 / R8347 |
| Price | | |
| Description | This kit enables enable detection of apoptosis in intact cell populations based on the measurement of caspase-3/7 activity using either a fluorescence microplate reader or a fluorescence imaging system. Caspase-3 and caspase-7 are proteases that are activated during the execution phase of apoptosis.This kit enables enable detection of apoptosis in intact cell populations based on the measurement of caspase-3/7 activity using either a fluorescence microplate reader or a fluorescence imaging system. Caspase-3 and caspase-7 are proteases that are activated during the execution phase of apoptosis. NucView 488 Caspase-3 substrate (formulated in DMSO or phosphate-buffered saline (PBS), for EarlyTox Caspase-3/7-D NucView 488 Assay Kit and EarlyTox Caspase-3/7 NucView 488 Assay Kit, respectively) is used to detect caspase-3/7 activity within intact cells without interfering with caspase activity. The substrate consists of a fluorogenic DNA dye that is coupled to the caspase-3/7 DEVD recognition sequence. Initially non-fluorescent, the substrate permeates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate, releasing a high-affinity DNA dye that migrates to the cell nucleus and stains DNA with bright green fluorescence, with excitation at 500 nm and emission at 530 nm. NucView 488 staining is formaldehyde-fixable and compatible with subsequent immunostaining. The assay kit includes an optional masking reagent to reduce background fluorescence when necessary. The PBS formulation is recommended for cells that are sensitive to DMSO.... Read More | This kit enables analysis of apoptosis based on caspase-3/7 activity and its consensus target sequence, Asp-Glu-Val-Asp (DEVD). Caspase-3 and caspase-7 are proteases that are activated during the execution phase of apoptosis. This kit provides a single-step, homogenous assay that is specifically This kit enables analysis of apoptosis based on caspase-3/7 activity and its consensus target sequence, Asp-Glu-Val-Asp (DEVD). Caspase-3 and caspase-7 are proteases that are activated during the execution phase of apoptosis. This kit provides a single-step, homogenous assay that is specifically designed for microplate readers. The fluorogenic substrate (Ac-DEVD)2-R110 contains two DEVD consensus target sequence and is completely hydrolyzed in cell lysate by the enzymes. Hydrolysis of both DEVD peptides releases the green fluorescent dye R110, resulting in a substantial fluorescence increase, with excitation at 490 nm and emission at 520 nm. The assay kit includes the competitive caspase-3/7 inhibitor Ac-DEVD-CHO for use as a negative control. R110 is also provided in the kit for generating a standard curve, which can be used for quantifying caspase-3 activity. Explorer Kit Components- 1 vial of Rhodamine 110 (80 µM, 1 mL)
- 2 vials of caspase-3 inhibitor (5 mM, 20 µL)
- 2 vials of DEVD-R110 (2 mM, 500 µL)
- 2 vials of lysis buffer (10 mL)
Each kit contains enough material for two 96-well or 384-well microplates.
Bulk Kit Components- 1 vial of Rhodamine 110 (80 µM, 1 mL)
- 2 vials of caspase-3 inhibitor (5 mM, 100 µL)
- 1 vial of DEVD-R110 (2 mM, 5 mL)
- 1 vial of lysis buffer (100 mL)
Each kit contains enough material for ten 96-well or 384-well microplates.... Read More |
| Detection Target | Caspase 3, 7 | Caspase 3, 7 |
| Method | Fluorescence Assay | Fluorescence Assay |
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