Assay kits are used to carry out analyses in a broad scope of applications including the study of targets such as drugs, molecules, disease pathways, chemical elements or compounds, and organisms. They are a routine procedure in the modern pharmaceutical, medical and forensic world as well as research and university laboratories around the globe. Assay kits come with all the necessary reagents, instructions and protocols for fast and efficient processing, allowing for repeatable results.
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| Company | Molecular Devices, LLC | Molecular Devices, LLC |
| Item | EarlyTox™ Caspase-3/7 NucView 488 Assay Kit | EarlyTox™ Glutathione Assay Kit |
| Catalog Number | R8350 / R8351 | R8344 / R8345 |
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| Description | This kit enables enable detection of apoptosis in intact cell populations based on the measurement of caspase-3/7 activity using either a fluorescence microplate reader or a fluorescence imaging system. Caspase-3 and caspase-7 are proteases that are activated during the execution phase of apoptosis.This kit enables enable detection of apoptosis in intact cell populations based on the measurement of caspase-3/7 activity using either a fluorescence microplate reader or a fluorescence imaging system. Caspase-3 and caspase-7 are proteases that are activated during the execution phase of apoptosis. NucView 488 Caspase-3 substrate (formulated in DMSO or phosphate-buffered saline (PBS), for EarlyTox Caspase-3/7-D NucView 488 Assay Kit and EarlyTox Caspase-3/7 NucView 488 Assay Kit, respectively) is used to detect caspase-3/7 activity within intact cells without interfering with caspase activity. The substrate consists of a fluorogenic DNA dye that is coupled to the caspase-3/7 DEVD recognition sequence. Initially non-fluorescent, the substrate permeates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate, releasing a high-affinity DNA dye that migrates to the cell nucleus and stains DNA with bright green fluorescence, with excitation at 500 nm and emission at 530 nm. NucView 488 staining is formaldehyde-fixable and compatible with subsequent immunostaining. The assay kit includes an optional masking reagent to reduce background fluorescence when necessary. The PBS formulation is recommended for cells that are sensitive to DMSO.... Read More | This kit enables the detection of an early apoptosis event based on the measurement of the glutathione content in live mammalian cells using a fluorescence microplate reader. Diminished cellular glutathione (GSH), which is an important anti-oxidant in mammalian cells, occurs at the early stages of This kit enables the detection of an early apoptosis event based on the measurement of the glutathione content in live mammalian cells using a fluorescence microplate reader. Diminished cellular glutathione (GSH), which is an important anti-oxidant in mammalian cells, occurs at the early stages of mitochondria-associated apoptosis because of GSH efflux. This kit uses monochlorobimane (MCB), a cell permeant dye that has a high affinity for GSH, to detect the cellular GSH level in an apoptotic event. The unreacted dye is almost non-fluorescent. After reaction of the dye with GSH, catalyzed by endogenous glutathione-S-transferase (GST) enzymes, the dye fluoresces blue, with excitation at 394 nm and emission at 490 nm. The intensity of the fluorescence signal generated from the assay reflects the amount of GSH that is present in the cells.... Read More |
| Detection Target | Caspase 3, 7 | Glutathione |
| Method | Fluorescence Assay | Fluorescence Assay |
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