When delivering smaller particles through the liquid mobile phase of a HPLC unit, the liquid must be forced through the column under pressure. The purpose of an HPLC pump, also called a solvent delivery system, is to force the liquid through the system while maintaining a specific flow rate. HPLC pumps can be broadly categorized into three types: syringe pumps that provide pulseless delivery, reciprocating or piston pumps that push the mobile phase through with a series of pistons, and pneumatic pumps that use gas to pressurize the mobile phase. Reciprocating pumps are most common due to their good pressure generation, constant flow rate, and small footprint, but each type of pump has its pros and cons that should be weighed when purchasing. Other things to look out for when selecting a pump include the instrument’s compatibility with different solvents, low dead volume to minimize issues with solvent changeover, delivery method (either isocratic or gradient), as well as things like price, ease of use, and lifetime.
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| Company | Shimadzu | Shimadzu |
| Item | LC-40B X3 HPLC/UHPLC Pump | i-Series LC-2080 |
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| Catalog Number | LC-40B X3 | 228-65883-58 |
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| Description | Boost your separation with Shimadzu's most powerful UHPLC pump. Our goal is to ensure low pulsation, gradient formation accuracy, and retention time reproducibility with our parallel dual plunger design. One LC-40BX3 can deliver two solvents for reliable high-pressure gradient separation. The designBoost your separation with Shimadzu's most powerful UHPLC pump. Our goal is to ensure low pulsation, gradient formation accuracy, and retention time reproducibility with our parallel dual plunger design. One LC-40BX3 can deliver two solvents for reliable high-pressure gradient separation. The design has no flow channel meeting point inside the pump, making it a true binary pump in a compact housing.
Features: - Built-in peristaltic pump washes behind the seals to minimize salt precipitation if buffered mobile phases are used.
- Unique FlowPilot technology ramps up the pump flow gradually, waits for the column to reach its set temperature, then ramps to the set flow rate, reducing potential column damage from pressure shock.
- The pump can display total operating time, volume of solvent delivered for each seal, and maintenance, error and operation logs.
- FlowPilot automatic diagnostic and recovery capabilities allow monitoring of pressure fluctuations for anomalies, restoration of normal operation, and can also alert users of low solvent levels.
- Users can set solvent compressibility to further reduce pulsation during operation.
- External valve option to supply 2 different solvents to each pump.
- Can be controlled by the LCD front panel keypad, web browser, and various HPLC and LC-MS/MS software.
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| Type | Parallel-type double plunger | UHPLC System (integrated, compact) |
| Flow Rate | 0.1 uL/min to 10 mL/min | 0.0001 to 10 mL/min |
| Gradient Type | High-pressure or quaternary low-pressure | Quaternary low-pressure gradient (LPG) |
| Number of Mixed Solvents | Inquire | 4 |
| Max. Pressure | 130 Mpa | Inquire |
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