When delivering smaller particles through the liquid mobile phase of a HPLC unit, the liquid must be forced through the column under pressure. The purpose of an HPLC pump, also called a solvent delivery system, is to force the liquid through the system while maintaining a specific flow rate. HPLC pumps can be broadly categorized into three types: syringe pumps that provide pulseless delivery, reciprocating or piston pumps that push the mobile phase through with a series of pistons, and pneumatic pumps that use gas to pressurize the mobile phase. Reciprocating pumps are most common due to their good pressure generation, constant flow rate, and small footprint, but each type of pump has its pros and cons that should be weighed when purchasing. Other things to look out for when selecting a pump include the instrument’s compatibility with different solvents, low dead volume to minimize issues with solvent changeover, delivery method (either isocratic or gradient), as well as things like price, ease of use, and lifetime.
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| Company | Shimadzu | Shimadzu |
| Item | i-Series LC-2080 | LC-40D HPLC/UHPLC Pump |
| Citations | | |
| Catalog Number | 228-65883-58 | LC-40D |
| Price | | |
| Description | Inquire | The easy to use and economically designed LC-40D pump enables reliable HPLC chromatography in isocratic, binary, or LPGE modes. The dual piston design has minimal pulsation for stable baselines.
Features: - Operable in constant flow or constant pressure mode, as well as isocratic, low pressure
The easy to use and economically designed LC-40D pump enables reliable HPLC chromatography in isocratic, binary, or LPGE modes. The dual piston design has minimal pulsation for stable baselines.
Features: - Operable in constant flow or constant pressure mode, as well as isocratic, low pressure gradient, ternary high-pressure gradient, or quaternary low-pressure gradient mode.
- Unique FlowPilot technology ramps up the pump flow gradually, waits for the column to reach its set temperature, then ramps to the set flow rate, reducing potential column damage from pressure shock.
- The pump can display total operating time, volume of solvent delivered for each seal, and maintenance, error and operation logs.
- Operable in constant flow or constant pressure mode, as well as isocratic, binary high-pressure gradient, ternary high-pressure gradient or quaternary low-pressure gradient mode.
- Internal solvent selection valve (2 or 4-channel) or a low-pressure gradient formation unit (LPGE) can be added as options.
- A rinse kit can be added to wash behind the seals to minimize salt precipitation if buffered mobile phases are used.
- Users can set compressibility to further reduce pulsation during operation.
- Can be controlled by the LCD front panel keypad, web browser, and various HPLC and LC-MS/MS software.
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| Type | UHPLC System (integrated, compact) | Parallel-type double plunger |
| Flow Rate | 0.0001 to 10 mL/min | 0.1 uL/min to 10 mL/min |
| Gradient Type | Quaternary low-pressure gradient (LPG) | High-pressure or quaternary low-pressure |
| Number of Mixed Solvents | 4 | Inquire |
| Max. Pressure | Inquire | 44 Mpa |
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