When delivering smaller particles through the liquid mobile phase of a HPLC unit, the liquid must be forced through the column under pressure. The purpose of an HPLC pump, also called a solvent delivery system, is to force the liquid through the system while maintaining a specific flow rate. HPLC pumps can be broadly categorized into three types: syringe pumps that provide pulseless delivery, reciprocating or piston pumps that push the mobile phase through with a series of pistons, and pneumatic pumps that use gas to pressurize the mobile phase. Reciprocating pumps are most common due to their good pressure generation, constant flow rate, and small footprint, but each type of pump has its pros and cons that should be weighed when purchasing. Other things to look out for when selecting a pump include the instrument’s compatibility with different solvents, low dead volume to minimize issues with solvent changeover, delivery method (either isocratic or gradient), as well as things like price, ease of use, and lifetime.
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| Company | Shimadzu | Shimadzu |
| Item | LC-20AP Large-scale Solvent Delivery Unit | LC-40D X3 HPLC/UHPLC Pump |
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| Catalog Number | LC-20AP | LC-40D X3 |
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| Description | The next generation LC-20AP Preparative LC Pump can be used for analytical, semi-prep and prep LC applications without changing pump heads.
The wide flow rate range (0.01 - 150mL/min) with high pressure tolerance (6,000 psi up to 100mL/min and 4,350 psi up to 150mL/min) makes it possible to use The next generation LC-20AP Preparative LC Pump can be used for analytical, semi-prep and prep LC applications without changing pump heads.
The wide flow rate range (0.01 - 150mL/min) with high pressure tolerance (6,000 psi up to 100mL/min and 4,350 psi up to 150mL/min) makes it possible to use 50mmID x 250mmL 5um-particle size columns to achieve high-resolution and high-efficiency purification, and improve productivity by five to ten times over the previous models. The flow rate accuracy and precision were drastically improved by minimizing dead volume in the pump heads and using higher resolution micro-stepping motor control so that the pump can be used at an analytical flow rate range, such as 1mL/min, with high reliability.
Overall productivity and quality of the purification has also been greatly improved. If configured as an analytical/prep system, a user can develop a method at analytical scale then immediately convert the method for prep scale. Shimadzu provides a tool to easily convert methods between analytical and prep for support customers. In addition, the LC-20AP pump can be used with Open Solution automated prep LC/MS software, which allows researchers to purify their compounds by simplifying the workflow in a walk-up environment.
In addition, the LC-20AP consumes 40% less energy in comparison with the previous model thanks to a newly designed high-efficiency power supply and motor control electronics. This makes the product more ecological.... Read More | Discover faster analysis, better resolution, and more reproducible chromatography with our new flagship UHPLC pump. Our industry leading LC-40D X3 pump ensures precision and accuracy at all flow rates.
Features: - Operable in constant flow or constant pressure mode, as well as isocratic, binary high
Discover faster analysis, better resolution, and more reproducible chromatography with our new flagship UHPLC pump. Our industry leading LC-40D X3 pump ensures precision and accuracy at all flow rates.
Features: - Operable in constant flow or constant pressure mode, as well as isocratic, binary high-pressure gradient, ternary high-pressure gradient, or quaternary low-pressure gradient mode.
- The pump can display total operating time, volume of solvent delivered for each seal, and maintenance, error and operation logs.
- Unique FlowPilot technology ramps up the pump flow gradually, waits for the column to reach its set temperature, then ramps to the set flow rate, reducing potential column damage from pressure shock.
- Users can set solvent compressibility to further reduce pulsation during operation.
- Can be controlled by the LCD front panel keypad, web browser, and various HPLC and LC-MS/MS software.
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| Type | Series-Type Double Plunger | Parallel-type double plunger |
| Plunger Capacity | 250 ul | Inquire |
| Flow Rate | 0.01 to 150.0 mL/min | 0.1 uL/min to 10 mL/min |
| Gradient Type | Constant-Pressure Solvent Delivery | High-pressure or quaternary low-pressure |
| Number of Mixed Solvents | Inquire | Inquire |
| Max. Pressure | 42.0 Mpa | 130 Mpa |
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