Polymerase chain reaction (PCR) is a workhorse technique of molecular biology laboratories in clinical and basic science. DNA thermal cyclers and gradient PCR units are essential in these laboratories. Selecting a good DNA thermal cycler is important. During PCR analysis, the well or tube capacity will determine the number of samples that can run at one time, including different combinations of temperature gradients (if available), MgCl2 concentrations, and primer levels within a single run. Temperature ranges, temperature ramp speeds, and programmability all influence an instrument’s versatility. PCR machines offer up to 384 well blocks, with temperatures reaching 100C. Some thermocyclers offer gradient capability and heated lids for more precise amplification protocols in PCR testing. The advantage of a PCR thermal cycler without gradient capability is low cost and applicability where thermal or concentration gradients are unnecessary.
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Eppendorf North America
- 4 °C to 99 °C
- 96- and 384-Wells
- 37 °C–110 °C
- Yes
NIPPON Genetics EUROPE
- 4 to 100 °C
- Inquire
- Heated, electronic, automatic
- Up to 6 °C per second
Analytik Jena
- 4 °C to 99 °C
- 9 or 384 well
- 30 °C to 110 °C
- Linear Gradient Tool
Analytik Jena
- 4 °C to 99 °C
- 96 and 384 well models
- can be set to temperatures of up to 110 °C
- Inquire
Analytik Jena
- 3 °C to 99 °C
- 96 and 384 well
- 30 °C–110 °C
- Yes, over 12 rows or 24 rows depending on model
Other DNA Thermal Cycler / PCR Instrument Products
Thermo Fisher Scientific
- Inquire
- Inquire
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Analytik Jena
- 3–99°C (block control range)
- 96-well (0.2 mL) or 384-well or 60-well (0.5 mL); interchangeable block modules
- Yes, 30–110°C (HPSL technology)
- Yes (G models); up to 30°C differential across 12 rows (20–99°C range)
Analytik Jena
- 3–99°C
- 96-well (0.2 mL); accepts tubes, 8-well strips, or 96-well microplates
- Yes, 30–110°C (High-Performance Smart Lid, HPSL)
- Yes (TOne 96 G model only); semi-linear gradient, 20°C max differential over 12 columns (20–99°C range)
Bio-Rad Laboratories
- 4–100°C (with standby to 4°C)
- 96-well (fast or deep well), 384-well, or dual 48/48-well via interchangeable reaction modules
- Yes, fully adjustable per module
- Yes (all reaction modules are gradient-enabled)
Inquire
Bio-Rad Laboratories
- 4–100°C
- 96 × 0.2 mL tubes, 0.2 mL tube strips, or 1 × 96-well plate
- Inquire
- Yes
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Eppendorf North America
- 4–99°C
- 64/32 × 0.2 mL PCR tubes or up to 24/10 × 0.5 mL PCR tubes (asymmetric dual block: 64-well + 32-well)
- Yes – flexlid® technology with thermal sample protection
- Yes – 1–12°C gradient range over 8 columns (0.2 mL block); gradient temperature control range 30–99°C
Eppendorf North America
- 4–99°C
- 64/32 × 0.2 mL PCR tubes or up to 24/10 × 0.5 mL PCR tubes (asymmetric dual block: 64-well + 32-well)
- Yes – flexlid® technology with thermal sample protection
- No – no gradient block; gradient is only available on the GX2 variant
Eppendorf North America
- 4 to 99 °C
- 96 x 0.2 mL PCR tubes, 1 PCR plate 8 x 12 or up to 71 x 0.5 mL PCR tubes
- Yes, 37-110 °C temp. range
- Yes
Qiagen
- 3°C to 99°C
- 96 x 0.2 mL tubes, 96-well microplates, or 8-well strips
- Yes
- Yes — Linear Gradient Tool, 20°C gradient span with 0.1°C increments
Hercuvan
- 4 - 100°C
- 96 wells (6 x 16 x 0.2 mL, 6 temperature zones)
- Yes, 30°C - 110°C
- Yes
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Opentrons
- 4 to 99 °C
- 96-well plate
- Yes, up to 110 °C
- Inquire
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- Expensive but Worth It
Eppendorf North America
Was considering the Mastercycler X50 vs a couple other cheaper options. Ultimately went with the X50 and we don't regret it at all. It's super easy to use, and is very easy to maintain. I've always ... Read Review