DNA Thermal Cycler / PCR Instrument

Polymerase chain reaction (PCR) is a workhorse technique of molecular biology laboratories in clinical and basic science. DNA thermal cyclers and gradient PCR units are essential in these laboratories. Selecting a good DNA thermal cycler is important. During PCR analysis, the well or tube capacity will determine the number of samples that can run at one time, including different combinations of temperature gradients (if available), MgCl2 concentrations, and primer levels within a single run. Temperature ranges, temperature ramp speeds, and programmability all influence an instrument’s versatility. PCR machines offer up to 384 well blocks, with temperatures reaching 100C. Some thermocyclers offer gradient capability and heated lids for more precise amplification protocols in PCR testing. The advantage of a PCR thermal cycler without gradient capability is low cost and applicability where thermal or concentration gradients are unnecessary.