Description | Demonstrating that cell lines are monoclonal – or that a gene was edited as expected – can be a time-consuming and highly-subjective process when relying on conventional technologies. The CloneSelect® Imager and CloneSelect® Imager FL are a high-throughput automated solutions for imaging and Demonstrating that cell lines are monoclonal – or that a gene was edited as expected – can be a time-consuming and highly-subjective process when relying on conventional technologies. The CloneSelect® Imager and CloneSelect® Imager FL are a high-throughput automated solutions for imaging and analyzing mammalian cells. Tracking the formation of a colony from a single cell is effortless as barcoded plates are tracked over time. Automated acquisition and analysis provides accurate, objective, and consistent results.
With high-resolution white light imaging, the CloneSelect Imager provides automated confluence, monoclonality assurance and industry leading acquisition times with the ability to image a 96-well plate in under two minutes.
The CloneSelect Imager FL features high contrast multichannel fluorescent and white light imaging that allows for accurate single cell detection and proof of monoclonality at day 0. Streamline your workflow with comparative confluence assays to identify and verify gene edits.
Features :
- White light and fluorescent imaging -
Image every well in every plate with rapid acquisition times with label-free white light. Multichannel fluorescence imaging provides additional confidence of monoclonality and comparative confluence assays (red vs green). -
Data tools to accelerate research timelines -
The software automatically calculates confluence measurements and generates growth curves, heatmaps, and image montages. Measurements for every well are automatically tracked over time. Streamline multiple steps (imaging, sample tracking, data analysis and report generation) in the cell line development workflow for IND filing. -
Quickly image a variety of plate formats and cell types -
Image a 96-well plate in under two minutes. Compatible with adherent or settled suspension cell types such as CHO, HEK, hybridomas, iPSCs, and many other cell types. -
Intelligent analysis with easy-to-use software -
The software automatically calculates confluence for each imaging time point. Growth curve, image montage, total growth, and mean rate are generated automatically and are exportable. Guided software user interface allows for simple 1-hour training. -
High-resolution images -
High-speed fluorescence and high-resolution white light imaging with accurate detection of single cells including debris. Image visualization and data tracking over multiple days. -
Custom automation options -
The Automation and Customization Team offers a variety of custom services from robotic plate loading to fully automated workstations with liquid handling and incubation. We have built out custom automated work cells with the CloneSelect Imager for iPSC workflows, full assay systems and drug toxicity and characterization research. ... Read More | Eight filter options provide simplified assay setup.
The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. The reader measures Eight filter options provide simplified assay setup.
The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to each reading.
Easy data visualization - Powerful curve fitting protocols and statistical analysis features are included for easy visualization of acquired data as grayscale or color map images, 3D graphs, kinetic plots, or reaction rates.
Compact design - The small footprint and low profile saves space on the bench.
Other features and benefits include: - Cover a wide range of applications
- Simplify assay setup
- Customize Options
... Read More | Filterless readers provide consistent results with steady temperature regulation.
The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Both Filterless readers provide consistent results with steady temperature regulation.
The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Both readers feature an 8-channel read head for fast reads and a subset feature our patented PathCheck™ Sensor. The temperature independent PathCheck sensor automatically normalizes and ensures accurate absorbance values by detecting pipetting errors. Combined with a wide application base, industry-leading SoftMax® Pro Software and validation tools, these readers fit in every lab and every budget.
Pipetting errors eliminated - This reader utilizes Molecular Devices' patented PathCheck® Sensor to measure the depth (optical pathlength) of samples in a microplate. It also allows for the elimination of standard curves.
Optimized optical path - The reader’s unique quartz fiber optic system minimizes stray light for precise readings across a microplate. The spectral resolution ensures accuracy of DNA absorbance measurements.
Eight-channel optics - The optical design of the reader mimics a dual-beam spectrophotometer. Each well has its own sample beam and reference detector. The eight-channel system delivers both precision and speed across the microplate.
Dual wavelength reads - The reader delivers high performance with round-bottom, flat-bottom or half-area well plates. Dual wavelength readings report the actual absorbance at each wavelength making data analysis convenient.
Flexibility to read a variety of plate formats - SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate.
Validation made easy - Verify the accuracy of your absorbance measurements with the SpectraTest® ABS1 Absorbance Validation Plate, using NIST-traceable standards. Testing can be done in your own lab, on your own schedule.
Other features and benefits include: - Eliminate filters
- Get consistent results
- Integrate with ease
... Read More | Flash and glow luminescence detection in an upgradeable platform.
The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold Flash and glow luminescence detection in an upgradeable platform.
The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume injector configuration make this reader ideal for fast kinetic assays requiring continuous monitoring during injection and measurement.
Reduced noise -
Ultra-fast photon counting technology amplifies the PMT signal with a fast pulse amplifier. This reduced noise capability enables a higher signal-to-noise ratio and low crosstalk.
PMT calibration -
The automatic PMT calibration method is based on readings of 4 internal LEDs at 395, 470, 527, and 570 nm. The manual PMT calibration method is based on readings of 4 wells from the assay.
Other features and benefits include:
- Detect all samples
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Get 2-fold improvement in speed
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Automate injector workflows
... Read More | Dual monochromators increase performance and assay flexibility.
The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Standard features include a cuvette port, spectral scanning in 1 nm Dual monochromators increase performance and assay flexibility.
The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. These robust readers have been placed in labs from Antarctica to the International Space Station.
With optimized reagents, validation tools, and industry-leading SoftMax® Pro Software, they provide consistent performance.
Patented optimization - The patented AutoPMT optimization of the SpectraMax M5 reader adjusts the fluorescence detector to each sample well's concentration and normalizes the raw data, extending the dynamic range of assays.
IQ/OQ/PM Services - Qualify Molecular Devices microplate readers in GLP or GMP environments with our IQ/OQ/PM Services to preserve the documentation of services in a compliant format that can be accessed remotely.
Validation tools - Extensive suite of validation tools reduces the cost and time of validation by 50% as compared to using multiple platforms to collect and analyze data.
Compliance software - SoftMax Pro GxP Software extends data acquisition and analysis solution into regulated laboratories working under GMP, GLP, and 21 CFR Part 11 for secure electronic records.
Other features and benefits include: - - Assay flexibility
- Better absorbance accuracy
- Increased throughput
... Read More |