| Description | Demonstrating that cell lines are monoclonal – or that a gene was edited as expected – can be a time-consuming and highly-subjective process when relying on conventional technologies. The CloneSelect® Imager and CloneSelect® Imager FL are a high-throughput automated solutions for imaging and Demonstrating that cell lines are monoclonal – or that a gene was edited as expected – can be a time-consuming and highly-subjective process when relying on conventional technologies. The CloneSelect® Imager and CloneSelect® Imager FL are a high-throughput automated solutions for imaging and analyzing mammalian cells. Tracking the formation of a colony from a single cell is effortless as barcoded plates are tracked over time. Automated acquisition and analysis provides accurate, objective, and consistent results.
With high-resolution white light imaging, the CloneSelect Imager provides automated confluence, monoclonality assurance and industry leading acquisition times with the ability to image a 96-well plate in under two minutes.
The CloneSelect Imager FL features high contrast multichannel fluorescent and white light imaging that allows for accurate single cell detection and proof of monoclonality at day 0. Streamline your workflow with comparative confluence assays to identify and verify gene edits.
Features :
- White light and fluorescent imaging -
Image every well in every plate with rapid acquisition times with label-free white light. Multichannel fluorescence imaging provides additional confidence of monoclonality and comparative confluence assays (red vs green). -
Data tools to accelerate research timelines -
The software automatically calculates confluence measurements and generates growth curves, heatmaps, and image montages. Measurements for every well are automatically tracked over time. Streamline multiple steps (imaging, sample tracking, data analysis and report generation) in the cell line development workflow for IND filing. -
Quickly image a variety of plate formats and cell types -
Image a 96-well plate in under two minutes. Compatible with adherent or settled suspension cell types such as CHO, HEK, hybridomas, iPSCs, and many other cell types. -
Intelligent analysis with easy-to-use software -
The software automatically calculates confluence for each imaging time point. Growth curve, image montage, total growth, and mean rate are generated automatically and are exportable. Guided software user interface allows for simple 1-hour training. -
High-resolution images -
High-speed fluorescence and high-resolution white light imaging with accurate detection of single cells including debris. Image visualization and data tracking over multiple days. -
Custom automation options -
The Automation and Customization Team offers a variety of custom services from robotic plate loading to fully automated workstations with liquid handling and incubation. We have built out custom automated work cells with the CloneSelect Imager for iPSC workflows, full assay systems and drug toxicity and characterization research.
... Read More | Unlimited breadth of user-upgradeable application modules expands research capabilities.
The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus Unlimited breadth of user-upgradeable application modules expands research capabilities.
The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes.
With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user-configurable detection modules, the reader provides you with the ability to explore cellular pathways and protein expression on one microplate reader.
User-configurable applications -
User-configurable detection modules expand the reader’s detection capabilities to include AlphaScreen, Time-Resolved Fluorescence, HTRF, fast kinetics with injectors, western blot detection, and more.
No-stain cell analysis -
The StainFree™ Cell Detection Algorithm for brightfield cell segmentation enables cell counting and confluency measurement without destructive stains, simplifying the cell counting workflow.
Multi-channel functionality -
Two fluorescence detection channels on the MiniMax cytometer enable the analysis of cell viability or cell toxicity assays, including ratiometric assays like live-dead and transfection efficiency.
Cellular pathway analysis -
Cell confluence and cell viability imaging, western blot analysis, and quantitation of nucleic acids and protein, can be captured on one reader.
Other features and benefits include:
- Capture flash assays with ease
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Reduce background noise
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Increase fluorescence performance
... Read More | NFC-based personalization and enhanced touchscreen workflows provide walk-up usability
The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET,NFC-based personalization and enhanced touchscreen workflows provide walk-up usability
The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection.
With optimized reagents, option to operate the readers using the touchscreen or a computer, and industry-leading SoftMax® Pro Software, the readers provide an all access pass for breakthrough research.
QuickSync -
The QuickSync feature pushes data to any computer within the same network and delivers data in an Excel-compatible format.
Flexible temperature control -
Simple-to-use temperature control adjusts the experiment's conditions from ambient up to 66°C.
Automatic filter identification -
Using NFC tags, the SpectraMax iD5 reader automatically detects the wavelength properties of each filter in addition to the filter slide configuration, eliminating confusion and the need for manual information entry.
Enhanced security -
The SoftMax® Touch Software secures user accounts with PIN- or NFC-protection with a lock screen option. Data is stored on the reader’s hard drive ensuring security even after transfer to a computer.
Hybrid optical system -
The SpectraMax iD5 reader provides the flexibility to set up experiments using filters, or a monochromator in 1 nm increments, or a hybrid mix of monochromator and filter-based wavelength selection.
Flash assays captured with ease -
The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 µL), overflow protection, and ensures equal mixing across the plate for high-precision experiments.
Other features and benefits include: - Personalize workflows
- • Simplify tasks
- • Reduce background noise
... Read More | Enables precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing consequence-free assay flexibility.
Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for Enables precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing consequence-free assay flexibility.
Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Narrow bandwidths deliver increased accuracy and enhanced peak resolution. Never order or replace filters to optimize an assay again!
A patented multichannel optical design mimics a dual-beam spectrophotometer, measures each sample in the plate directly, and eliminates error due to variations in light output between optical fibers on other instruments for more reliable reporting. The eight-channel system delivers high precision and speed when reading 96-well microplates for single endpoint measurements and kinetic assay data gathered over time.
The VersaMax Microplate Reader is supplied with SoftMax® Pro Data Acquistion & Analysis Software, Molecular Devices' industry leading all-in-one data acquisition and analysis software. Additionally, the VersaMax Microplate Reader can be seamlessly integrated with the StakMax® Microlate Handling Stacker through the SoftMax Pro Software.
Features - Enabling Assays without Compromise
- Measuring Accurately and Reproducibly
- Comprehensive Reader Validation
- Automating with Ease
... Read More | The VMax® Kinetic ELISA Microplate Reader, the first of its kind for monitoring reactions over time, opened the door to enzyme assays and continues to be the workhorse of the laboratory environment. Based on a rugged, simple design, the VMax Absorbance Microplate Reader offers a mean time between The VMax® Kinetic ELISA Microplate Reader, the first of its kind for monitoring reactions over time, opened the door to enzyme assays and continues to be the workhorse of the laboratory environment. Based on a rugged, simple design, the VMax Absorbance Microplate Reader offers a mean time between failure (MTBF) of 20,000 hours with 97 independent and stationary fiber optics so that the microplate remains stationary during the entire reading cycle, reducing the number of moving parts that can wear and need replacement.
Through sequential, single-well illumination, 100% of the available light is directed to each well through the optical fibers to achieve linearity to higher optical densities (ODs) and optimal signal-to-noise ratios. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance.
The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. Additional high-quality filters to supplement your assay wavelength range requirements are available directly from Molecular Devices.
VMax Microplate Readers can be operated as stand-alone instruments and are also supplied with SoftMax® Pro Data Acquistion & Analysis Software, Molecular Devices' industry leading all-in-one data acquisition and analysis software.
Features - Extend uptime with an estimated 20,000 hour mean time between failure
- Maximize assay signal-to-noise ratios through a unique optical system that directs all available light to each well
- Achieve higher throughput with a 5 second microplate read time
- Read up to 2 wavelengths in endpoint or kinetic absorbance for 96-well plates
- Mix samples in microplate wells by user-programmable Automix feature
- Operate without an attached computer using the onboard microprocessor
- Add assay flexibility with additional filters
... Read More |