Microplate readers read biological, chemical or physical events that occur on a microtiter plate. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. This makes these readers a staple in research labs specializing in drug discovery, bioassay validation, manufacturing, etc...
Considerations for purchasing a microplate reader
The first thing to consider when purchasing a microplate reader is the detection method. Microplates typically measure absorbance, fluorescence intensity, or luminescence, but models that measure time-resolved fluorescence and fluorescence polarization are also available for purchase. The type of detection to use will depend on the application. ELISAs and protein assays, for example, require absorbance to be measured while luciferase-based gene expression assays will require luminescence to be measured.
Other things to consider include plate format – 96-well and 384-well plates are the norm but higher and lower capacity plate sizes are also available, speed and throughput needed, type of software, and whether or not multiple plates or wells can be read simultaneously.
Find, compare, and request a quote for microplate readers across leading suppliers at Labcompare; including access to product citations, related published figures, and first-hand reviews from our scientific community.
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Company | Agilent Technologies | Agilent Technologies |
Item | Agilent BioTek Cytation 5 Cell Imaging Multimode Reader | Agilent BioTek Synergy H1 Multimode Reader |
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Catalog Number | Cytation 5 | Synergy H1 |
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Description | The Agilent BioTek Cytation 5 combines automated microscopy and conventional microplate detection in a configurable, upgradable platform. The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield, and phase contrast to address The Agilent BioTek Cytation 5 combines automated microscopy and conventional microplate detection in a configurable, upgradable platform. The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield, and phase contrast to address many applications and workflows. The multimode detection modules include filter- and monochromator-based fluorescence detection, luminescence, and UV-Vis absorbance detection. Gen5 software provides complete control over all imaging and data capture, plus powerful image and data analysis.... Read More | Agilent BioTek Synergy H1 is a modular multimode microplate reader, with monochromator-based optics and filter-based optics. The patented Hybrid Technology offers flexibility and sensitivity across a broad range of applications. The modular platform allows upgrading to expand functionality as your Agilent BioTek Synergy H1 is a modular multimode microplate reader, with monochromator-based optics and filter-based optics. The patented Hybrid Technology offers flexibility and sensitivity across a broad range of applications. The modular platform allows upgrading to expand functionality as your laboratory workflows change. Synergy H1 offers continuously variable bandwidth monochromators for fluorescence excitation and emission wavelength selection. Fluorescence bandwidth can be set between 9 and 50 nm, in 1 nm increments, allowing users to fully optimize reader settings to drive the best assay performance compared to fixed bandwidth systems.... Read More |
Optical System | Monochromator system,
Filter system | Monochromator or Filter/Dichroics-based |
Measuring Time | 11 s / 96 wells,
22 s / 384 wells | 11 to 42 s (varies with wells) |
Wavelength Range | 200 to 850 nm | 230 to 999 nm (depending on model) |
Plate Formats | 6 to 384-well plates or 6 to 1536-well plates | 1 to 384 Well Plates |
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