| Description | Multi-Link™ Position Control is a stand-alone software package that interfaces with our MPC-200 manipulator controllers. Multi-Link is an intuitive GUI that uses the MPC-200 driver Sutter developed for the NIH freeware, µManager. It operates any Sutter Instrument device that can be connected to the Multi-Link™ Position Control is a stand-alone software package that interfaces with our MPC-200 manipulator controllers. Multi-Link is an intuitive GUI that uses the MPC-200 driver Sutter developed for the NIH freeware, µManager. It operates any Sutter Instrument device that can be connected to the MPC-200, including: manipulators, motorized microscopes (MOM and SOM), the MT-800 translator (with or without focus drive), and the MPC-78 platform stage (with or without focus drive) and the 3DMS stage.
The most powerful aspect of the Multi-Link software is the ability to link the movement of multiple devices together. The lead pipette can be used to direct the movement of a microscope translator (objective/camera), or the translator can function as the leader and thereby keep the pipettes in the field of view. The leading device can be controlled through the manual input knobs of the ROE-200 or the computer GUI via a mouse or other PC interface.
Multi-Link easily memorizes multiple working locations and can rapidly switch between them. The user simply moves the microscope to a region of interest and clicks to save the location. Turning on “Link” will then coordinate all linked devices (usually manipulators) with moves made by the microscope via a motorized translator. Switching between memorized positions will bring all linked devices to that location. Link, intuitive and easy to use, will allow users to bring all their pipettes to a working location in seconds! The memory positions available in the Multi- Link software allow for most of the same robotic functionality found in our MP-285. Memorized locations can be repeated in looped operation.
While all features found in the ROE-200 have been replicated in Multi-Link, full functionality of the ROE is retained, allowing movement of any connected device by turning the knobs on the ROE or by clicking and dragging the pipettes in software. Moves made in the GUI update the positional information displayed on the ROE, and moves made with the ROE-200 are updated in Multi-Link.... Read More | ScanImage is imaging software for scanning microscopes developed by Karel Svoboda and coworkers (Pologruto et al, 2003) and judiciously maintained by members of the Svoboda lab, first at CSHL and then at Janelia Farms, HHMI. It is hard to over-estimate the influence of this software in the field of ScanImage is imaging software for scanning microscopes developed by Karel Svoboda and coworkers (Pologruto et al, 2003) and judiciously maintained by members of the Svoboda lab, first at CSHL and then at Janelia Farms, HHMI. It is hard to over-estimate the influence of this software in the field of two-photon scanning microscopy.
ScanImage was designed to be freely available and open source and its early maintenance was “easily” supported. However, as ScanImage became increasingly popular and as new versions were required to cover changes in operating systems and hardware drivers, it soon became obvious that a high level of maintenance could not be sustained based on the “freeware model”. Ultimately, ScanImage support and development has become a commercial enterprise and Vidrio is the new face of ScanImage.
Two-photon microscope development at Sutter Instrument has been closely tied to ScanImage software for years. In addition to the MOM® in vivo two-photon scope, Sutter has many products that are used by two-photon microscopists using ScanImage including our stepper motor drive XYZ controllers, galvo and resonant scanner systems, PMTs and PMT power supplies. We are grateful to have had a mutual, continuing connection to ScanImage and the ScanImage community for over ten years.
Sutter provides hardware that operates seamlessly with all versions of ScanImage. Products can be purchased ala carte or as a bundled system with ScanImage Premium or SI 5.... Read More | SutterPatch® software is a full-featured electrophysiology data acquisition, management and analysis application for Windows or Mac OS computers. SutterPatch comes bundled with all Sutter Instrument Patch Clamp Amplifier Systems. The software controls data acquisition, provides real-time SutterPatch® software is a full-featured electrophysiology data acquisition, management and analysis application for Windows or Mac OS computers. SutterPatch comes bundled with all Sutter Instrument Patch Clamp Amplifier Systems. The software controls data acquisition, provides real-time measurements to aid decision making during the experiment, keeps track of all amplifier parameters, records the experimental progress and stores a set of up to 500 metadata parameters. Built within the latest version of Igor Pro by WaveMetrics, Inc., SutterPatch provides immediate access to Igor’s powerful scientific and engineering analysis tools.
Version 2 of SutterPatch software adds support for the new dPatch® Digital Patch Amplifier System as well as a multitude of new features and user interface improvements that also apply to the IPA Family of Amplifier Systems. The Membrane Test and Free Run have been overhauled. New triple slider and 2D-matrix controls facilitate compensation adjustments in the Amplifier Control Panel.
Particular emphasis was put into intuitive navigation through large data sets. Controls that are familiar from electrophysiology software or applications in other fields, as well as entirely new approaches make finding a particular section of an experiment very easy.
The structured architecture of the data files was designed to retain the context of every sample within an experiment. With little effort at the beginning of an experiment, a plethora of metadata parameters is recorded – automatically where possible, configured by the user where desired. Each parameter can be reviewed before a Paradigm or Routine is executed.
SutterPatch software comes with a collection of sample Paradigms and Routines that help configuring commonly executed experimental scenarios.
SutterPatch provides real-time analysis capability that creates graphs like I-V curves or a time course plot while the experiment is in progress. Up to 8 analysis graphs can be shown, each derived from 16 possible measurements from the input signals. Analyses include mean, slope, rise time, frequency of threshold crossing, etc. This facilitates making decisions about the further course of an experiment.
In addition to real-time analysis, further processing after the experiment allows performing the most sophisticated analysis procedures. SutterPatch adds application-specific capabilities on top of the expansive analysis feature set that is native to Igor Pro. Equations and Variables facilitate the use of more complex algorithms in both Routines and Paradigms.... Read More |