Ultra High Performance Liquid Chromatograph (UHPLC)

Ultra high-performance liquid chromatography, UHPLC, uses columns packed with porous sub-2-µm particles (usually 1.8 µm) to vastly increase throughput and resolving power compared to conventional 5-µm columns. Pressures can surpass 19,000 psi. Limited solvent consumption, the ability to work under a broader pressure range, and lower band broadening are further enhancements. UHPLC’s 10× reduction in analysis time strengthens its popularity in areas including the pharmaceutical industry, food safety, and the clinical field. The choice of detectors for use with UHPLC is wide-ranging, including UV-VIS, fluorescence, photodiode array, ELSD, quadrupole, and most recently, refractive index. For flexibility, many instruments offer the ability to run both conventional and UHPLC on one instrument. A final tip: When making the switch from HPLC to UHPLC, resist the temptation to overload the smaller columns.

Discover and compare Ultra High Performance Liquid Chromatographs:

CompanyShimadzuShimadzu
ItemNexera X3 UHPLC SystemNexera XR UHPLC System
Citations
Catalog NumberNexera X3Nexera XR
Price
DescriptionNexera X3 excels in high throughput environments requiring fast separations. The system easily handles small ID UHPLC columns with sub-2 micron particles for high efficiency chromatography. Five-minute run times are the norm. With Analytical Intelligence functions, the system makes lab management ... Read MoreNexera XR is perfect for fast separations using the popular SPP column phases for high efficiency and lower back pressure. This system offers a rugged and forgiving UHPLC workflow, with five to ten-minute separations. With Analytical Intelligence functions, the system makes lab management simple, ... Read More
QuantityEAEA
Detector(s)UV, PDA, ELSD, Fluorescence, RID, ConductivityUV, PDA, ELSD, Fluorescence, RID, Conductivity
Flow Rate0.0001 – 3 mL/min (1.0 – 130 MPa); 3 – 5 mL/min (1.0 – 80 MPa); 5 – 10 mL/min (1.0 – 22 MPa)0.0001 – 3 mL/min (1.0 – 70 MPa); 3 – 5 mL/min (1.0 – 44 MPa); 5 – 10 mL/min (1.0 – 22 MPa)
Max. Pressure130 Mpa70 Mpa
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