Ultra High Performance Liquid Chromatograph (UHPLC)

Ultra high-performance liquid chromatography, UHPLC, uses columns packed with porous sub-2-µm particles (usually 1.8 µm) to vastly increase throughput and resolving power compared to conventional 5-µm columns. Pressures can surpass 19,000 psi. Limited solvent consumption, the ability to work under a broader pressure range, and lower band broadening are further enhancements. UHPLC’s 10× reduction in analysis time strengthens its popularity in areas including the pharmaceutical industry, food safety, and the clinical field. The choice of detectors for use with UHPLC is wide-ranging, including UV-VIS, fluorescence, photodiode array, ELSD, quadrupole, and most recently, refractive index. For flexibility, many instruments offer the ability to run both conventional and UHPLC on one instrument. A final tip: When making the switch from HPLC to UHPLC, resist the temptation to overload the smaller columns.

Discover and compare Ultra High Performance Liquid Chromatographs:

CompanyShimadzuShimadzu
ItemNexera XS inert UHPLCNexera UHPLC Method Scouting
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Catalog NumberNexera BioinertNexera Method Scouting
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DescriptionA truly bioinert UHPLC, the Nexera XS inert has been engineered with unique technology that ensures the complete inertness of the sample path to eliminate corrosion and adsorption in bioanalysis, hence providing newfound clarity even for challenging compounds. This reduces sample losses and enables ... Read MoreMethod scouting is a process where different mobile phases and column stationary phases are evaluated to determine the best set of separation conditions for a particular sample. Manual solvent preparation, column switching, and method programming are time-consuming steps, so the Nexera method ... Read More
QuantityEAEA
Detector(s)UV, PDA, ELSD, FluorescenceSPD-M20A
Flow Rate0.0001 – 3.0000 mL/min (1.0 – 105 MPa); 3.0001 – 5.0000 mL/min (1.0 – 80 MPa); 5.0001 – 10.0000 mL/min (1.0 – 22 MPa)Inquire
Max. Pressure105 Mpa100 Mpa
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