Ultra High Performance Liquid Chromatograph (UHPLC)

Ultra high-performance liquid chromatography, UHPLC, uses columns packed with porous sub-2-µm particles (usually 1.8 µm) to vastly increase throughput and resolving power compared to conventional 5-µm columns. Pressures can surpass 19,000 psi. Limited solvent consumption, the ability to work under a broader pressure range, and lower band broadening are further enhancements. UHPLC’s 10× reduction in analysis time strengthens its popularity in areas including the pharmaceutical industry, food safety, and the clinical field. The choice of detectors for use with UHPLC is wide-ranging, including UV-VIS, fluorescence, photodiode array, ELSD, quadrupole, and most recently, refractive index. For flexibility, many instruments offer the ability to run both conventional and UHPLC on one instrument. A final tip: When making the switch from HPLC to UHPLC, resist the temptation to overload the smaller columns.

Discover and compare Ultra High Performance Liquid Chromatographs:

CompanyShimadzuShimadzuShimadzuShimadzuShimadzu
ItemNexera UCs UHPLC/SFC Switcing SystemNexera X3 UHPLC SystemNexera X4 UHPLC SystemNexera XS inert UHPLCNexera XS UHPLC System
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Catalog NumberNexera UC SwitchingNexera X3Nexera BioinertNexera XS
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DescriptionThis system can switch automatically between SFC analysis and UHPLC analysis and make measurements on a single sample in each separation mode. It enhances user-friendliness and efficiency by allowing the investigation of separation conditions and performing reverse-phase high-speed analysis in a ... Read MoreNexera X3 excels in high throughput environments requiring fast separations. The system easily handles small ID UHPLC columns with sub-2 micron particles for high efficiency chromatography. Five-minute run times are the norm. With Analytical Intelligence functions, the system makes lab management ... Read MoreInquireA truly bioinert UHPLC, the Nexera XS inert has been engineered with unique technology that ensures the complete inertness of the sample path to eliminate corrosion and adsorption in bioanalysis, hence providing newfound clarity even for challenging compounds. This reduces sample losses and enables ... Read MoreThe Nexera XS UHPLC excels at fast separations, easily handling small ID UHPLC columns with sub-2 micron particles or the popular SPP phases for high efficiency and lower back pressure. A common XS configuration may have a 4-channel solvent selection valve on each pump providing versatility for ... Read More
QuantityEAEAInquireEAEA
Detector(s)UV, PDAUV, PDA, ELSD, Fluorescence, RID, ConductivityPhotodiode array detector (PDA)UV, PDA, ELSD, FluorescenceUV, PDA, ELSD, Fluorescence, RID, Conductivity
Flow RateInquire0.0001 – 3 mL/min (1.0 – 130 MPa); 3 – 5 mL/min (1.0 – 80 MPa); 5 – 10 mL/min (1.0 – 22 MPa)0.0001–2.0 mL/min0.0001 – 3.0000 mL/min (1.0 – 105 MPa); 3.0001 – 5.0000 mL/min (1.0 – 80 MPa); 5.0001 – 10.0000 mL/min (1.0 – 22 MPa)0.0001 – 3 mL/min (1.0 – 105 MPa); 3 – 5 mL/min (1.0 – 80 MPa); 5 – 10 mL/min (1.0 – 22 MPa)
Max. PressureInquire130 Mpa130 MPa105 Mpa105 Mpa
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