Ultra High Performance Liquid Chromatograph (UHPLC)

Ultra high-performance liquid chromatography, UHPLC, uses columns packed with porous sub-2-µm particles (usually 1.8 µm) to vastly increase throughput and resolving power compared to conventional 5-µm columns. Pressures can surpass 19,000 psi. Limited solvent consumption, the ability to work under a broader pressure range, and lower band broadening are further enhancements. UHPLC’s 10× reduction in analysis time strengthens its popularity in areas including the pharmaceutical industry, food safety, and the clinical field. The choice of detectors for use with UHPLC is wide-ranging, including UV-VIS, fluorescence, photodiode array, ELSD, quadrupole, and most recently, refractive index. For flexibility, many instruments offer the ability to run both conventional and UHPLC on one instrument. A final tip: When making the switch from HPLC to UHPLC, resist the temptation to overload the smaller columns.

Discover and compare Ultra High Performance Liquid Chromatographs:

CompanyAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent Technologies
ItemAgilent 1290 Infinity III LC SystemAgilent 1260 Infinity III Bio-Inert LC SystemAgilent 1260 Infinity III LC SystemAgilent 1260 Infinity III Prime Bio LC SystemAgilent 1260 Infinity III Prime LC System
Price
Detector(s)DAD, FLD, RID, MSDUV, FLD, RIDDAD HS, DAD WR, VWD, MWD, FLD, FLD Spectra, RID, ELSD, MSDAD, VWD, FLD, RID, ELSD, MSDAD, VWD, FLD, RID, ELSD, MS
Flow Rate0.05–5 mL/min0.2–10 mL/min0.001–10 mL/minup to 5 mL/minup to 5 mL/min
Max. Pressure1300 bar600 bar (8700 psi)600 bar (8702 psi)800 bar800 bar (11603 psi)
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