Ultra High Performance Liquid Chromatograph (UHPLC)

Ultra high-performance liquid chromatography, UHPLC, uses columns packed with porous sub-2-µm particles (usually 1.8 µm) to vastly increase throughput and resolving power compared to conventional 5-µm columns. Pressures can surpass 19,000 psi. Limited solvent consumption, the ability to work under a broader pressure range, and lower band broadening are further enhancements. UHPLC’s 10× reduction in analysis time strengthens its popularity in areas including the pharmaceutical industry, food safety, and the clinical field. The choice of detectors for use with UHPLC is wide-ranging, including UV-VIS, fluorescence, photodiode array, ELSD, quadrupole, and most recently, refractive index. For flexibility, many instruments offer the ability to run both conventional and UHPLC on one instrument. A final tip: When making the switch from HPLC to UHPLC, resist the temptation to overload the smaller columns.

Discover and compare Ultra High Performance Liquid Chromatographs:

CompanyThermo Fisher ScientificThermo Fisher ScientificThermo Fisher ScientificThermo Fisher ScientificThermo Fisher Scientific
ItemThermo Scientific Vanquish Trap Heart-Cut 2D-LC SystemsThermo Scientific Vanquish Core HPLC SystemThermo Scientific Vanquish Loop Heart-Cut 2D-LC SystemsThermo Scientific Vanquish Simple Switch 2D-LC SystemsThermo Scientific Vanquish Tandem LC Systems
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Detector(s)Charged Aerosol Detector (CAD), Diode Array Detector (DAD), Fluorescence Detector (FLD), Multiple Wavelength Detector (MWD), Variable Wavelength Detector (VWD), Refractive Index Detector (RI), Mass SpectrometerCharged Aerosol Detector (CAD), Diode Array Detector (DAD), Fluorescence Detector (FLD), Multiple Wavelength Detector (MWD), Variable Wavelength Detector (VWD), Refractive Index Detector (RI), Mass SpectrometerCharged Aerosol Detector (CAD), Diode Array Detector (DAD), Fluorescence Detector (FLD), Multiple Wavelength Detector (MWD), Variable Wavelength Detector (VWD), Refractive Index Detector (RI), Mass SpectrometerCharged Aerosol Detector (CAD), Diode Array Detector (DAD), Fluorescence Detector (FLD), Multiple Wavelength Detector (MWD), Variable Wavelength Detector (VWD), Refractive Index Detector (RI), Mass SpectrometerCharged Aerosol Detector (CAD), Diode Array Detector (DAD), Fluorescence Detector (FLD), Multiple Wavelength Detector (MWD), Variable Wavelength Detector (VWD), Refractive Index Detector (RI), Mass Spectrometer
Flow Rate0–5 or 0–8 mL/min (depends on pumps selected)0–10 mL/min0–5, 0–8, or 0–10 mL/min (depends on pumps selected)0–5 or 0–8 mL/min (depends on pumps selected)0–5, 0–8, or 0–10 mL/min (depends on pumps selected)
Max. Pressure1500 bar700 bar700–1500 bar (depends on configuration)1000 or 1500 bar (depends on configuration)700, 1000, or 1500 bar (depends on configuration)
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