Ultra High Performance Liquid Chromatograph (UHPLC)

Ultra high-performance liquid chromatography, UHPLC, uses columns packed with porous sub-2-µm particles (usually 1.8 µm) to vastly increase throughput and resolving power compared to conventional 5-µm columns. Pressures can surpass 19,000 psi. Limited solvent consumption, the ability to work under a broader pressure range, and lower band broadening are further enhancements. UHPLC’s 10× reduction in analysis time strengthens its popularity in areas including the pharmaceutical industry, food safety, and the clinical field. The choice of detectors for use with UHPLC is wide-ranging, including UV-VIS, fluorescence, photodiode array, ELSD, quadrupole, and most recently, refractive index. For flexibility, many instruments offer the ability to run both conventional and UHPLC on one instrument. A final tip: When making the switch from HPLC to UHPLC, resist the temptation to overload the smaller columns.

Discover and compare Ultra High Performance Liquid Chromatographs:

CompanyShimadzuShimadzuShimadzuShimadzuShimadzu
ItemCBM-40 HPLC/UHPLC System ControllerNexera UHPLC Method ScoutingNexera X3 UHPLC SystemNexera XS inert UHPLCSCL-40 HPLC/UHPLC System Controller
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Catalog NumberCBM-40Nexera Method ScoutingNexera X3Nexera BioinertSCL-40
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DescriptionThe CBM-40 is a small-profile unit that stacks neatly with main system components. Other LC modules connect via fiber optic cables for easy "plug and play" system configuration. An internal web server offers convenient control and monitoring of the UHPLC system through a web browser, LabSolutions ... Read MoreMethod scouting is a process where different mobile phases and column stationary phases are evaluated to determine the best set of separation conditions for a particular sample. Manual solvent preparation, column switching, and method programming are time-consuming steps, so the Nexera method ... Read MoreNexera X3 excels in high throughput environments requiring fast separations. The system easily handles small ID UHPLC columns with sub-2 micron particles for high efficiency chromatography. Five-minute run times are the norm. With Analytical Intelligence functions, the system makes lab management ... Read MoreA truly bioinert UHPLC, the Nexera XS inert has been engineered with unique technology that ensures the complete inertness of the sample path to eliminate corrosion and adsorption in bioanalysis, hence providing newfound clarity even for challenging compounds. This reduces sample losses and enables ... Read MoreThe SCL-40 features a built-in reservoir tray and color LCD touch screen, stacking neatly on top of the main system components. Other LC modules connect via fiber optic cables for easy "plug and play" system configuration. An internal web server offers convenient control and monitoring of the UHPLC ... Read More
QuantityEAEAEAEAEA
Detector(s)InquireSPD-M20AUV, PDA, ELSD, Fluorescence, RID, ConductivityUV, PDA, ELSD, FluorescenceInquire
Flow RateInquireInquire0.0001 – 3 mL/min (1.0 – 130 MPa); 3 – 5 mL/min (1.0 – 80 MPa); 5 – 10 mL/min (1.0 – 22 MPa)0.0001 – 3.0000 mL/min (1.0 – 105 MPa); 3.0001 – 5.0000 mL/min (1.0 – 80 MPa); 5.0001 – 10.0000 mL/min (1.0 – 22 MPa)Inquire
Max. PressureInquire100 Mpa130 Mpa105 MpaInquire
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