Ultra High Performance Liquid Chromatograph (UHPLC)

Ultra high-performance liquid chromatography, UHPLC, uses columns packed with porous sub-2-µm particles (usually 1.8 µm) to vastly increase throughput and resolving power compared to conventional 5-µm columns. Pressures can surpass 19,000 psi. Limited solvent consumption, the ability to work under a broader pressure range, and lower band broadening are further enhancements. UHPLC’s 10× reduction in analysis time strengthens its popularity in areas including the pharmaceutical industry, food safety, and the clinical field. The choice of detectors for use with UHPLC is wide-ranging, including UV-VIS, fluorescence, photodiode array, ELSD, quadrupole, and most recently, refractive index. For flexibility, many instruments offer the ability to run both conventional and UHPLC on one instrument. A final tip: When making the switch from HPLC to UHPLC, resist the temptation to overload the smaller columns.

Discover and compare Ultra High Performance Liquid Chromatographs:

CompanyShimadzuShimadzuShimadzuShimadzuShimadzu
ItemNexera UCs UHPLC/SFC Switcing SystemNexera UHPLC Method ScoutingNexera X3 UHPLC SystemNexera XR UHPLC SystemNexera XS UHPLC System
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Catalog NumberNexera UC SwitchingNexera Method ScoutingNexera X3Nexera XRNexera XS
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DescriptionThis system can switch automatically between SFC analysis and UHPLC analysis and make measurements on a single sample in each separation mode. It enhances user-friendliness and efficiency by allowing the investigation of separation conditions and performing reverse-phase high-speed analysis in a ... Read MoreMethod scouting is a process where different mobile phases and column stationary phases are evaluated to determine the best set of separation conditions for a particular sample. Manual solvent preparation, column switching, and method programming are time-consuming steps, so the Nexera method ... Read MoreNexera X3 excels in high throughput environments requiring fast separations. The system easily handles small ID UHPLC columns with sub-2 micron particles for high efficiency chromatography. Five-minute run times are the norm. With Analytical Intelligence functions, the system makes lab management ... Read MoreNexera XR is perfect for fast separations using the popular SPP column phases for high efficiency and lower back pressure. This system offers a rugged and forgiving UHPLC workflow, with five to ten-minute separations. With Analytical Intelligence functions, the system makes lab management simple, ... Read MoreThe Nexera XS UHPLC excels at fast separations, easily handling small ID UHPLC columns with sub-2 micron particles or the popular SPP phases for high efficiency and lower back pressure. A common XS configuration may have a 4-channel solvent selection valve on each pump providing versatility for ... Read More
QuantityEAEAEAEAEA
Detector(s)InquireSPD-M20AUV, PDA, ELSD, Fluorescence, RID, ConductivityUV, PDA, ELSD, Fluorescence, RID, ConductivityUV, PDA, ELSD, Fluorescence, RID, Conductivity
Flow RateInquireInquire0.0001 – 3 mL/min (1.0 – 130 MPa); 3 – 5 mL/min (1.0 – 80 MPa); 5 – 10 mL/min (1.0 – 22 MPa)0.0001 – 3 mL/min (1.0 – 70 MPa); 3 – 5 mL/min (1.0 – 44 MPa); 5 – 10 mL/min (1.0 – 22 MPa)0.0001 – 3 mL/min (1.0 – 105 MPa); 3 – 5 mL/min (1.0 – 80 MPa); 5 – 10 mL/min (1.0 – 22 MPa)
Max. PressureInquire100 Mpa130 Mpa70 Mpa105 Mpa
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