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| Company | Waters | Wyatt Technology | Waters | Wyatt Technology | Waters | Wyatt Technology |
| Item | DAWN™ Multi-angle Light Scattering (MALS) Instrument | DynaPro Plate Reader 4 Dynamic Light Scattering Instrument | microDAWN™ Multi-Angle Light Scattering Instrument for UHP-SEC-MALS |
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| Catalog Number | DAWN | DynaPro Plate Reader 4 | microDAWN |
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| Description | The DAWN™ instrument is an advanced detector for precise characterization of proteins, polymers, and nanoparticles including viral vectors, lipid nanoparticles and vesicles. Using multi-angle static light scattering (MALS) and optional dynamic light scattering (DLS), DAWN analyzes molar massThe DAWN™ instrument is an advanced detector for precise characterization of proteins, polymers, and nanoparticles including viral vectors, lipid nanoparticles and vesicles. Using multi-angle static light scattering (MALS) and optional dynamic light scattering (DLS), DAWN analyzes molar mass, size, conformation, particle concentration, conjugation (e.g. glycoproteins) or encapsulation (e.g. AAV capsid content). It stands out with exceptional sensitivity and the widest range of molecular weight, size, and concentration of any MALS instrument, paired with a broad selection of configurations and optional modules for enhanced capabilities.
The DAWN is most often used in conjunction with gel permeation chromatography (GPC-MALS), size exclusion chromatography (SEC-MALS) or an EclipseTM, field-flow fractionation instrument (FFF-MALS) and may also be used with ion-exchange chromatography or reverse-phase chromatography. The instrument includes a high-resolution, touch-screen front panel to display data graphs, system health and instrument diagnostics and is accompanied by ASTRA™, the most versatile software package available for analysis of online multi-angle and dynamic light scattering.... Read More | The DynaPro Plate Reader 4 is a high-throughput DLS instrument that delivers rapid measurements of particle size, concentration, aggregation, and stability of many different macromolecular or nanoparticle samples. It enables fully-automated screening of processing or formulation conditions - simply The DynaPro Plate Reader 4 is a high-throughput DLS instrument that delivers rapid measurements of particle size, concentration, aggregation, and stability of many different macromolecular or nanoparticle samples. It enables fully-automated screening of processing or formulation conditions - simply load and walk away. Measurements are completed in standard 96, 384 or 1536 disposable well plates and the instrument can be integrated with plate and liquid-handling robots for additional time savings.... Read More | The microDAWN™ MALS detector performs absolute characterization of macromolecules eluting from UHPLC (or UPLC), determining molar mass and size independently of retention time and molecular reference standards. This instrument offers superb sensitivity over a wide range of molar mass, size andThe microDAWN™ MALS detector performs absolute characterization of macromolecules eluting from UHPLC (or UPLC), determining molar mass and size independently of retention time and molecular reference standards. This instrument offers superb sensitivity over a wide range of molar mass, size and concentrations. In most respects, the microDAWN is similar to the well-established miniDAWN, a workhorse MALS instrument used in hundreds of labs around the world for characterizing proteins and other molecules smaller than 50 nm in radius. The microDAWN is uniquely suitable for use in conjunction with UHPLC Size Exclusion Chromatography (µSEC-MALS) thanks to its small interdetector dispersion and produces minimal band broadening in order to maintain the narrow peaks typical of UHPLC. In some cases it may be used with ion-exchange or other types of chromatography.... Read More |
| Type | MALS and optional DLS | Dynamic Light Scattering / Static Light Scattering | MALS and optional DLS |
| Particle Size | Inquire | 0.5 to 1000 nm | Inquire |
| Detector(s) | High-gain, high-dynamic-range photodiodes at 18 angles for MALS; optional avalanche photodiode that may be placed at any of four angles for DLS. Fluorescence-blocking filters available for use with analytes that fluoresce when illuminated at the MALS wavelength. | Linearized avalanche photodiode for dynamic and static light scattering | High-gain, high dynamic range, static light scattering detectors at 3 angles |
| Measuring Time | Depedent on LC separation | Measurement Time: 5 to 60 seconds per well 830 nm | Depedent on LC separation |
| Light Source | 658 nm laser, programmable 10% - 100% of maximum power.
Optional: 785 nm for fluorescence suppression | 830 nm, fiber-coupled diode laser | 660 nm laser, programmable 10% - 100% of maximum power. |
| Applications | With standard SEC/GPC or EclipseTM (FFF system): determination of distributions of molar mass, size, conformation, conjugation ratio and particle concentration.
With Calypso composition-gradient system: label-free characterization of biomolecular interactions including self- and hetero-association as well as non-specific interactions. | Develop stable biopharmaceuticals, assess viral vector titer and aggregation, optimize protein buffers for crystallization, SAXS or chromatography, formulate theranostic nanoparticles, discover small-molecule and peptide inhibitors of protein-protein interactions | With SEC/GPC: determination of distributions of absolute molar mass, size, conformation, conjugate ratio. |
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