Liquid chromatography-mass spectroscopy (LCMS) requires extensive sample preparation in order to achieve its famed precision and accuracy. An LCMS sample preparation system can use membranes with various type of filters, such as hydrophilic nylon or polyethersulfone filters; alternatively, an HPLC sample preparation system can utilize vacuum purification. LCMS sample preparation systems can handle sample volumes as small as 100 µl, while others can handle <100 µl and >100 µl capacity. LCMS sample preparation equipment such as kits are also available.
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| Company | Shimadzu | Shimadzu | Shimadzu |
| Item | Perfinity Integrated Digestion Platform (iDP) | CLAM-2030 Sample Preparation Module for LCMS | Perfinity Workstation |
| Citations | | | |
| Catalog Number | Perfinity iDP | CLAM-2030 | Perfinity |
| Price | | | |
| Description | The Perfinity integrated Digestion Platform (iDP) automates key proteomics workflow steps including protein digestion, desalting and reversed phase chromatography to significantly reduce sample preparation times and enhance reproducibility. The automated process removes much of the equipment and The Perfinity integrated Digestion Platform (iDP) automates key proteomics workflow steps including protein digestion, desalting and reversed phase chromatography to significantly reduce sample preparation times and enhance reproducibility. The automated process removes much of the equipment and labor associated with protein sample preparation for LCMS analyses, minimizing a potential source of error and improving laboratory productivity. Additionally, the Perfinity trypsin immobilized enzyme reaction (IMER) columns reduce digestion times from eighteen hours to between one and eight minutes.
Features
- Reproducible protein digestions with CV’s < 10%
- 1-8 minute trypsin digestions
- Fully automated proteolysis workflow
- Ability to couple directly to any LC/MS
Perfinity iDP can be used in multiple application areas, including assay development, protein purification, drug development, and biomarker discovery.... Read More | The Clinical Laboratory Automation Module (CLAM-2030) is a fully automated sample preparation module for LCMS designed to improve reproducibility of test results, while increasing laboratory efficiency. Simply place blood collection or other tubes in the sample rack and the system automatically The Clinical Laboratory Automation Module (CLAM-2030) is a fully automated sample preparation module for LCMS designed to improve reproducibility of test results, while increasing laboratory efficiency. Simply place blood collection or other tubes in the sample rack and the system automatically performs everything from sample pretreatment through to the LCMS analysis. The CLAM-2030 essentially facilitates the ability for any level of user to reproducibly prepare samples and operate an LCMS regardless of their skill level. With a streamlined workflow that interfaces with LIS, users can easily create and run sample batches with minimal effort. STAT or Emergency Sample functionality allows users to prioritize urgent samples for quick results with minimal interruption.
The system also offers a variety of functions that elevate research and business efficiency, as it automates processes including searching toxicological drug libraries, providing the optimal analytical method, facilitating data review and generating reports.
Key Benefits
• Seamless Integration of Sample Preparation and LCMS Analysis
• Precise Control of Analytical Results
• Excellent Data Accuracy and Reproducibility
• Eliminates the Need to Review Settings for Routine Work
• Can Measure Hundreds of Compounds in a Single Analysis
• Reduces Operator Errors
• Enhances Laboratory Safety During Procedures... Read More | Perfinity Workstation, developed in partnership with Perfinity Biosciences Inc., is a multi-column apparatus that automates protein separations and mass spec sample preparation for more efficient LC/MS analysis. Unlike 2D LC systems, this platform utilizes the concept of ‘digital elution’ to link Perfinity Workstation, developed in partnership with Perfinity Biosciences Inc., is a multi-column apparatus that automates protein separations and mass spec sample preparation for more efficient LC/MS analysis. Unlike 2D LC systems, this platform utilizes the concept of ‘digital elution’ to link various sample preparation processes. Targeted analytes bound in one column are forwarded on to the next process. The Perfinity Workstation utilizes Shimadzu hardware components in conjunction with five Perfinity optimized columns that each perform a step of the mass-spec sample-preparation process -affinity selection, buffer exchange, digestion, desalting and reverse-phase separation. The automated integration of these steps enables users to start with serum and have peptides ready for LC/MS analysis in 10 minutes. The automated process removes much of the equipment and labor associated with mass-spec analyses of proteins. Key benefits include: - Serum to purified peptides in < 10="" min.="" />
- Hands free operation – fully automated
- Significant reduction in cost per sample
- Easily customizable
The major advantage of the Perfinity Workstation’s sample-preparation approach is that users are not required to immobilize antibodies. Instead, scientists can add antibodies directly to the sample. By removing the immobilization process, users have eliminated a potential source of error, making LC/MS analysis with Perfinity Workstation more accurate and reliable. This platform is customizable across several application areas, including assay development, protein purification, drug development and biomarker discovery. *Available in the U.S. only... Read More |
| Type | Digestion Platform | Inquire | Inquire |
| Sample Volume | Inquire | Inquire | Inquire |
| Capacity | 200 samples/day | Inquire | Inquire |
| Speed | 1 to 4 mins (Trypsin digestion) | Inquire | Inquire |
| Dimensions | Inquire | Inquire | Inquire |
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