HPLC Instrument

High-performance liquid chromatography (HPLC) (also called high-pressure liquid chromatography) is a solution-phase technique for fractionation of complex samples. Unlike standard column chromatography, HPLC is an active process in which materials are pumped at high pressure through the separation column, which contains the so-called “stationary phase” of the separation in the form of densely packed, often chemically functionalized beads. Once introduced to the column, it is the sample’s differential affinity for the solvent (“mobile”) and stationary phases that enables separation to occur, generally with sharper resolution than standard liquid chromatography. High performance liquid chromatographs can be used for analytical or preparative applications depending on the HPLC pump and the column size. HPLC columns are available in a variety of formats, including reverse phase, ion exchange, and size exclusion, which separate based on polarity, charge, and size, respectively.

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CompanyShimadzuShimadzu
ItemLC-40 HPLC Systemi-Series Integrated (u)HPLC Systems
Citations
Catalog NumberLC-40i-Series LC-70/80
Price
TypeHPLCAnalytical
Detector(s)UV, PDA, ELSD, Fluorescence, RID, ConductivityUV/VIS, PDA, RI, Fluorescence, ELSD, LCMS
Flow Rate0.0001 – 5 mL/min (1.0 – 44 MPa); 5 – 10 mL/min (1.0 – 22 MPa)0.0001 to 10 mL/min
Max. Pressure44 Mpa70 MPa
DescriptionThe modular LC-40 system is the workhorse for everyday HPLC, whether using traditional 5 micron fully porous LC columns or the popular SPP column phases for high efficiency separations with lower back pressures. With Analytical Intelligence functions, the system makes lab management simple, enabling... Read MoreBuilt for fast and accurate sample analysis, the latest iteration of Shimadzu’s compact, robust integrated (U)HPLC system maintains the excellent core performance capabilities of its predecessor while incorporating innovative functionality to drive productivity and ensure high-quality analytical ... Read More
QuantityEAEA
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