HPLC Instrument

High-performance liquid chromatography (HPLC) (also called high-pressure liquid chromatography) is a solution-phase technique for fractionation of complex samples. Unlike standard column chromatography, HPLC is an active process in which materials are pumped at high pressure through the separation column, which contains the so-called “stationary phase” of the separation in the form of densely packed, often chemically functionalized beads. Once introduced to the column, it is the sample’s differential affinity for the solvent (“mobile”) and stationary phases that enables separation to occur, generally with sharper resolution than standard liquid chromatography. High performance liquid chromatographs can be used for analytical or preparative applications depending on the HPLC pump and the column size. HPLC columns are available in a variety of formats, including reverse phase, ion exchange, and size exclusion, which separate based on polarity, charge, and size, respectively.

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CompanyShimadzuShimadzuShimadzuShimadzuShimadzu
Itemi-Series Integrated (u)HPLC Systems2D HPLC for Bioanalysis (Co-Sense for BA)Amino Acid Analysis SystemCannabis Analyzer for PotencyLC-40 HPLC System
Citations
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Catalog Numberi-Series LC-70/80Co-Sense for BAAmino Acid HPLCCannabis AnalyzerLC-40
Price
TypeAnalyticalAnalyticalAmino Acid Analysis SystemInquireHPLC
Detector(s)UV/VIS, PDA, RI, Fluorescence, ELSD, LCMSUV, LCMSPost-column fluorescence derivatizationInquireUV, PDA, ELSD, Fluorescence, RID, Conductivity
Flow Rate0.0001 to 10 mL/minInquireInquireInquire0.0001 – 5 mL/min (1.0 – 44 MPa); 5 – 10 mL/min (1.0 – 22 MPa)
Max. Pressure70 MPaInquireInquireInquire44 Mpa
DescriptionBuilt for fast and accurate sample analysis, the latest iteration of Shimadzu’s compact, robust integrated (U)HPLC system maintains the excellent core performance capabilities of its predecessor while incorporating innovative functionality to drive productivity and ensure high-quality analytical ... Read MoreAutomatically and seamlessly conducting all processes from sample pre-treatment to analysis, the Prominence 2D HPLC for BA is a column-switching HPLC system equipped with the newly developed Shim-pack MAYI-ODS pretreatment column and a unique on-line dilution bypass channel. The system’s on-line pre... Read MoreThe analysis of amino acids plays an important role not only in the food industry, but also in natural product and pharmaceutical fields. This system uses detection by post-column fluorescence derivatization, with o-phthalaldehyde (OPA) / N-acetylcysteine as the reaction reagent, to selectively and ... Read MoreThe Shimadzu Cannabis Analyzer for Potency captures the spirit of an Analyzer – a comprehensive package integrating instrument hardware, software, consumables, and analytical workflow. Includes a certified reference material (CRM) mixture of target compounds. Also includes a service package to cover... Read MoreThe modular LC-40 system is the workhorse for everyday HPLC, whether using traditional 5 micron fully porous LC columns or the popular SPP column phases for high efficiency separations with lower back pressures. With Analytical Intelligence functions, the system makes lab management simple, enabling... Read More
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