HPLC Instrument

High-performance liquid chromatography (HPLC) (also called high-pressure liquid chromatography) is a solution-phase technique for fractionation of complex samples. Unlike standard column chromatography, HPLC is an active process in which materials are pumped at high pressure through the separation column, which contains the so-called “stationary phase” of the separation in the form of densely packed, often chemically functionalized beads. Once introduced to the column, it is the sample’s differential affinity for the solvent (“mobile”) and stationary phases that enables separation to occur, generally with sharper resolution than standard liquid chromatography. High performance liquid chromatographs can be used for analytical or preparative applications depending on the HPLC pump and the column size. HPLC columns are available in a variety of formats, including reverse phase, ion exchange, and size exclusion, which separate based on polarity, charge, and size, respectively.

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CompanyShimadzuShimadzu
Itemi-Series Integrated (u)HPLC SystemsNexera UHPLC Method Scouting
Citations
(1)
Catalog Numberi-Series LC-70/80Nexera Method Scouting
Price
TypeAnalyticalUHPLC
Detector(s)UV/VIS, PDA, RI, Fluorescence, ELSD, LCMSSPD-M20A
Flow Rate0.0001 to 10 mL/minInquire
Max. Pressure70 MPa100 Mpa
DescriptionBuilt for fast and accurate sample analysis, the latest iteration of Shimadzu’s compact, robust integrated (U)HPLC system maintains the excellent core performance capabilities of its predecessor while incorporating innovative functionality to drive productivity and ensure high-quality analytical ... Read MoreMethod scouting is a process where different mobile phases and column stationary phases are evaluated to determine the best set of separation conditions for a particular sample. Manual solvent preparation, column switching, and method programming are time-consuming steps, so the Nexera method ... Read More
QuantityEAEA
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