HPLC Instrument

High-performance liquid chromatography (HPLC) (also called high-pressure liquid chromatography) is a solution-phase technique for fractionation of complex samples. Unlike standard column chromatography, HPLC is an active process in which materials are pumped at high pressure through the separation column, which contains the so-called “stationary phase” of the separation in the form of densely packed, often chemically functionalized beads. Once introduced to the column, it is the sample’s differential affinity for the solvent (“mobile”) and stationary phases that enables separation to occur, generally with sharper resolution than standard liquid chromatography. High performance liquid chromatographs can be used for analytical or preparative applications depending on the HPLC pump and the column size. HPLC columns are available in a variety of formats, including reverse phase, ion exchange, and size exclusion, which separate based on polarity, charge, and size, respectively.

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CompanyAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent Technologies
Item1290 Infinity III LC System1260 Infinity III Amino Acid Analysis System1260 Infinity III Prime Bio Analytical-Scale LC Purification System1290 Infinity III Bio LC System1290 Infinity III High-Throughput System
Price
TypeUHPLCHPLCInquireBio UHPLCHigh-Throughput UHPLC
Detector(s)VWD, DAD, FLD, RID, ELSD, LC/MSDAD, FLDInquireVWD, DAD, FLD, Bio MDS, LC/MSDAD with Max-Light cartridge, LC/MS compatible
Flow Rate0.001-5 mL/minInquire0.001-5 mL/minup to 5 mL/minup to 5 mL/min
Max. Pressure1300 bar600 bar800 bar1300 bar1300 bar
DescriptionAnalytical UHPLC system with market-leading sample capacity, binary and quaternary pump options, up to 1300 bar, InfinityLab Assist technology.Complete automated HPLC amino acid analysis system.Biocompatible HPLC/UHPLC purification system for analytical-scale bio applications, up to 800 bar.Biocompatible UHPLC system with binary or quaternary options, up to 1300 bar, for biopharma applications.High-throughput UHPLC system with up to 16 microplates (6144 samples), 1300 bar, 5-second cycle times.
CapacityInquireInquireInquireInquireUp to 16 cooled microplates (6,144 samples)
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