HPLC Instrument

High-performance liquid chromatography (HPLC) (also called high-pressure liquid chromatography) is a solution-phase technique for fractionation of complex samples. Unlike standard column chromatography, HPLC is an active process in which materials are pumped at high pressure through the separation column, which contains the so-called “stationary phase” of the separation in the form of densely packed, often chemically functionalized beads. Once introduced to the column, it is the sample’s differential affinity for the solvent (“mobile”) and stationary phases that enables separation to occur, generally with sharper resolution than standard liquid chromatography. High performance liquid chromatographs can be used for analytical or preparative applications depending on the HPLC pump and the column size. HPLC columns are available in a variety of formats, including reverse phase, ion exchange, and size exclusion, which separate based on polarity, charge, and size, respectively.

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CompanyAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent Technologies
Item1290 Infinity III LC System1260 Infinity III Bio-Inert Analytical-Scale LC Purification System1260 Infinity III SFC/UHPLC Hybrid System1290 Infinity III Analytical-Scale LC Purification System1290 Infinity III Bio LC System
Price
TypeUHPLCInquireInquireAnalytical-Scale Preparative UHPLCBio UHPLC
Detector(s)VWD, DAD, FLD, RID, ELSD, LC/MSInquireInquireDAD (240 Hz), RI, FLD, ELSD, LC/MSD optionalVWD, DAD, FLD, Bio MDS, LC/MS
Flow Rate0.001-5 mL/min0.001-10 mL/minUp to 5 mL/minup to 5 mL/minup to 5 mL/min
Max. Pressure1300 bar600 bar600 bar (SFC) / 800 bar (UHPLC)1300 bar1300 bar
DescriptionAnalytical UHPLC system with market-leading sample capacity, binary and quaternary pump options, up to 1300 bar, InfinityLab Assist technology.Bio-inert HPLC purification system for large biomolecules, up to 600 bar.Hybrid SFC/UHPLC system with automated mode switching. 600 bar SFC, 800 bar UHPLC.Analytical-scale UHPLC purification system for flow rates up to 5 mL/min, columns up to 10 mm id.Biocompatible UHPLC system with binary or quaternary options, up to 1300 bar, for biopharma applications.
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