HPLC Instrument

High-performance liquid chromatography (HPLC) (also called high-pressure liquid chromatography) is a solution-phase technique for fractionation of complex samples. Unlike standard column chromatography, HPLC is an active process in which materials are pumped at high pressure through the separation column, which contains the so-called “stationary phase” of the separation in the form of densely packed, often chemically functionalized beads. Once introduced to the column, it is the sample’s differential affinity for the solvent (“mobile”) and stationary phases that enables separation to occur, generally with sharper resolution than standard liquid chromatography. High performance liquid chromatographs can be used for analytical or preparative applications depending on the HPLC pump and the column size. HPLC columns are available in a variety of formats, including reverse phase, ion exchange, and size exclusion, which separate based on polarity, charge, and size, respectively.

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CompanyAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent Technologies
Item1260 Infinity III LC System1260 Infinity III SFC/UHPLC Hybrid System1290 Infinity III Bio LC System1290 Infinity III Bio Online LC System1290 Infinity III Online SPE System
Price
TypeModular HPLC/UHPLCInquireBio UHPLCInquireInquire
Detector(s)VWD, DAD, FLD, RID, ELSDInquireVWD, DAD, FLD, Bio MDS, LC/MSInquireInquire
Flow Rate0.001-10 mL/minUp to 5 mL/minup to 5 mL/minup to 5 mL/min0.001-5 mL/min
Max. Pressure600 bar (8700 psi)600 bar (SFC) / 800 bar (UHPLC)1300 bar1300 bar1300 bar
DescriptionRobust analytical HPLC system with binary, quaternary, or isocratic pump options, up to 600 bar. InfinityLab Assist technology.Hybrid SFC/UHPLC system with automated mode switching. 600 bar SFC, 800 bar UHPLC.Biocompatible UHPLC system with binary or quaternary options, up to 1300 bar, for biopharma applications.Biocompatible online UHPLC system for real-time bioprocess monitoring with up to 1300 bar.Automated online solid phase extraction UHPLC system for analyte enrichment and matrix removal.
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