HPLC Instrument

High-performance liquid chromatography (HPLC) (also called high-pressure liquid chromatography) is a solution-phase technique for fractionation of complex samples. Unlike standard column chromatography, HPLC is an active process in which materials are pumped at high pressure through the separation column, which contains the so-called “stationary phase” of the separation in the form of densely packed, often chemically functionalized beads. Once introduced to the column, it is the sample’s differential affinity for the solvent (“mobile”) and stationary phases that enables separation to occur, generally with sharper resolution than standard liquid chromatography. High performance liquid chromatographs can be used for analytical or preparative applications depending on the HPLC pump and the column size. HPLC columns are available in a variety of formats, including reverse phase, ion exchange, and size exclusion, which separate based on polarity, charge, and size, respectively.

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CompanyAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent Technologies
Item1260 Infinity III Prime Bio LC System1260 Infinity III SFC System1290 Infinity III Analytical-Scale LC Purification System1290 Infinity III Bio Analytical-Scale LC Purification System1290 Infinity III Method Development System
Price
TypeBio HPLC/UHPLCInquireAnalytical-Scale Preparative UHPLCInquireInquire
Detector(s)Bio-Inert VWD, DAD, FLDInquireDAD (240 Hz), RI, FLD, ELSD, LC/MSD optionalInquireInquire
Flow Rate0.001-5 mL/minUp to 5 mL/minup to 5 mL/minup to 5 mL/minUp to 5 mL/min
Max. Pressure800 bar (11600 psi)600 bar1300 bar1300 bar1300 bar
DescriptionVersatile bio HPLC/UHPLC system for bioseparations with biocompatible flow path and quaternary pump up to 800 bar.Supercritical fluid chromatography system, up to 600 bar at flow rates up to 5 mL/min.Analytical-scale UHPLC purification system for flow rates up to 5 mL/min, columns up to 10 mm id.Biocompatible UHPLC purification system for multimilligram quantities, up to 1300 bar.UHPLC method development system with automated column and solvent switching, up to 1300 bar.
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