HPLC Instrument

High-performance liquid chromatography (HPLC) (also called high-pressure liquid chromatography) is a solution-phase technique for fractionation of complex samples. Unlike standard column chromatography, HPLC is an active process in which materials are pumped at high pressure through the separation column, which contains the so-called “stationary phase” of the separation in the form of densely packed, often chemically functionalized beads. Once introduced to the column, it is the sample’s differential affinity for the solvent (“mobile”) and stationary phases that enables separation to occur, generally with sharper resolution than standard liquid chromatography. High performance liquid chromatographs can be used for analytical or preparative applications depending on the HPLC pump and the column size. HPLC columns are available in a variety of formats, including reverse phase, ion exchange, and size exclusion, which separate based on polarity, charge, and size, respectively.

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CompanyAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent Technologies
Item1260 Infinity III Prime Bio LC System1260 Infinity III Method Development System1260 Infinity III Prime Analytical-Scale LC Purification System1290 Infinity III Bio LC System1290 Infinity III Bio Online LC System
Price
TypeBio HPLC/UHPLCInquireInquireBio UHPLCInquire
Detector(s)Bio-Inert VWD, DAD, FLDInquireInquireVWD, DAD, FLD, Bio MDS, LC/MSInquire
Flow Rate0.001-5 mL/min0.001-10 mL/min0.001-5 mL/minup to 5 mL/minup to 5 mL/min
Max. Pressure800 bar (11600 psi)600 bar800 bar1300 bar1300 bar
DescriptionVersatile bio HPLC/UHPLC system for bioseparations with biocompatible flow path and quaternary pump up to 800 bar.Automated HPLC method development system with column and solvent switching.Flexible preparative HPLC/UHPLC system for analytical-scale purification, up to 800 bar.Biocompatible UHPLC system with binary or quaternary options, up to 1300 bar, for biopharma applications.Biocompatible online UHPLC system for real-time bioprocess monitoring with up to 1300 bar.
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