HPLC Instrument

High-performance liquid chromatography (HPLC) (also called high-pressure liquid chromatography) is a solution-phase technique for fractionation of complex samples. Unlike standard column chromatography, HPLC is an active process in which materials are pumped at high pressure through the separation column, which contains the so-called “stationary phase” of the separation in the form of densely packed, often chemically functionalized beads. Once introduced to the column, it is the sample’s differential affinity for the solvent (“mobile”) and stationary phases that enables separation to occur, generally with sharper resolution than standard liquid chromatography. High performance liquid chromatographs can be used for analytical or preparative applications depending on the HPLC pump and the column size. HPLC columns are available in a variety of formats, including reverse phase, ion exchange, and size exclusion, which separate based on polarity, charge, and size, respectively.

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CompanyAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent Technologies
Item1260 Infinity III Prime Bio LC System1260 Infinity III Analytical-Scale LC Purification System1260 Infinity III Method Development System1290 Infinity III Method Development System1290 Infinity III Online SPE System
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TypeBio HPLC/UHPLCInquireInquireInquireInquire
Detector(s)Bio-Inert VWD, DAD, FLDInquireInquireInquireInquire
Flow Rate0.001-5 mL/min0.001-10 mL/min0.001-10 mL/minUp to 5 mL/min0.001-5 mL/min
Max. Pressure800 bar (11600 psi)600 bar600 bar1300 bar1300 bar
DescriptionVersatile bio HPLC/UHPLC system for bioseparations with biocompatible flow path and quaternary pump up to 800 bar.Analytical-scale HPLC purification system for preparative work up to 600 bar.Automated HPLC method development system with column and solvent switching.UHPLC method development system with automated column and solvent switching, up to 1300 bar.Automated online solid phase extraction UHPLC system for analyte enrichment and matrix removal.
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