HPLC Instrument

High-performance liquid chromatography (HPLC) (also called high-pressure liquid chromatography) is a solution-phase technique for fractionation of complex samples. Unlike standard column chromatography, HPLC is an active process in which materials are pumped at high pressure through the separation column, which contains the so-called “stationary phase” of the separation in the form of densely packed, often chemically functionalized beads. Once introduced to the column, it is the sample’s differential affinity for the solvent (“mobile”) and stationary phases that enables separation to occur, generally with sharper resolution than standard liquid chromatography. High performance liquid chromatographs can be used for analytical or preparative applications depending on the HPLC pump and the column size. HPLC columns are available in a variety of formats, including reverse phase, ion exchange, and size exclusion, which separate based on polarity, charge, and size, respectively.

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CompanyAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent Technologies
Item1260 Infinity III Amino Acid Analysis System1260 Infinity III Analytical-Scale LC Purification System1290 Infinity III Bio LC System1290 Infinity III Bio Online LC System1290 Infinity III High-Throughput System
Price
TypeHPLCInquireBio UHPLCInquireHigh-Throughput UHPLC
Detector(s)DAD, FLDInquireVWD, DAD, FLD, Bio MDS, LC/MSInquireDAD with Max-Light cartridge, LC/MS compatible
Flow RateInquire0.001-10 mL/minup to 5 mL/minup to 5 mL/minup to 5 mL/min
Max. Pressure600 bar600 bar1300 bar1300 bar1300 bar
DescriptionComplete automated HPLC amino acid analysis system.Analytical-scale HPLC purification system for preparative work up to 600 bar.Biocompatible UHPLC system with binary or quaternary options, up to 1300 bar, for biopharma applications.Biocompatible online UHPLC system for real-time bioprocess monitoring with up to 1300 bar.High-throughput UHPLC system with up to 16 microplates (6144 samples), 1300 bar, 5-second cycle times.
CapacityInquireInquireInquireInquireUp to 16 cooled microplates (6,144 samples)
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