HPLC Instrument

High-performance liquid chromatography (HPLC) (also called high-pressure liquid chromatography) is a solution-phase technique for fractionation of complex samples. Unlike standard column chromatography, HPLC is an active process in which materials are pumped at high pressure through the separation column, which contains the so-called “stationary phase” of the separation in the form of densely packed, often chemically functionalized beads. Once introduced to the column, it is the sample’s differential affinity for the solvent (“mobile”) and stationary phases that enables separation to occur, generally with sharper resolution than standard liquid chromatography. High performance liquid chromatographs can be used for analytical or preparative applications depending on the HPLC pump and the column size. HPLC columns are available in a variety of formats, including reverse phase, ion exchange, and size exclusion, which separate based on polarity, charge, and size, respectively.

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CompanyAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent Technologies
Item1290 Infinity III Bio LC System1260 Infinity III Amino Acid Analysis System1260 Infinity III Prime Bio LC System1290 Infinity III Multi-Method System1290 Infinity III Online SPE System
Price
TypeBio UHPLCHPLCBio HPLC/UHPLCInquireInquire
Detector(s)VWD, DAD, FLD, Bio MDS, LC/MSDAD, FLDBio-Inert VWD, DAD, FLDInquireInquire
Flow Rateup to 5 mL/minInquire0.001-5 mL/minUp to 5 mL/min0.001-5 mL/min
Max. Pressure1300 bar600 bar800 bar (11600 psi)1300 bar1300 bar
DescriptionBiocompatible UHPLC system with binary or quaternary options, up to 1300 bar, for biopharma applications.Complete automated HPLC amino acid analysis system.Versatile bio HPLC/UHPLC system for bioseparations with biocompatible flow path and quaternary pump up to 800 bar.UHPLC multi-method system for multiple users with automated method switching, up to 1300 bar.Automated online solid phase extraction UHPLC system for analyte enrichment and matrix removal.
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