HPLC Instrument

High-performance liquid chromatography (HPLC) (also called high-pressure liquid chromatography) is a solution-phase technique for fractionation of complex samples. Unlike standard column chromatography, HPLC is an active process in which materials are pumped at high pressure through the separation column, which contains the so-called “stationary phase” of the separation in the form of densely packed, often chemically functionalized beads. Once introduced to the column, it is the sample’s differential affinity for the solvent (“mobile”) and stationary phases that enables separation to occur, generally with sharper resolution than standard liquid chromatography. High performance liquid chromatographs can be used for analytical or preparative applications depending on the HPLC pump and the column size. HPLC columns are available in a variety of formats, including reverse phase, ion exchange, and size exclusion, which separate based on polarity, charge, and size, respectively.

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CompanyAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent Technologies
Item1290 Infinity III Bio LC System1260 Infinity III Bio-Inert Analytical-Scale LC Purification System1260 Infinity III Method Development System1290 Infinity III 2D-LC System1290 Infinity III HDR-DAD Impurity Analyzer System
Price
TypeBio UHPLCInquireInquire2D-LC UHPLCInquire
Detector(s)VWD, DAD, FLD, Bio MDS, LC/MSInquireInquireMultiple detector configurationsHDR-DAD
Flow Rateup to 5 mL/min0.001-10 mL/min0.001-10 mL/minup to 5 mL/minup to 5 mL/min
Max. Pressure1300 bar600 bar600 bar1300 barup to 1300 bar
DescriptionBiocompatible UHPLC system with binary or quaternary options, up to 1300 bar, for biopharma applications.Bio-inert HPLC purification system for large biomolecules, up to 600 bar.Automated HPLC method development system with column and solvent switching.Two-dimensional LC UHPLC system for comprehensive separations and MS compatibility. Up to 1300 bar.Impurity analyzer with 30x higher dynamic range and UV sensitivity. UHPLC resolution at up to 1300 bar.
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