HPLC Instrument

High-performance liquid chromatography (HPLC) (also called high-pressure liquid chromatography) is a solution-phase technique for fractionation of complex samples. Unlike standard column chromatography, HPLC is an active process in which materials are pumped at high pressure through the separation column, which contains the so-called “stationary phase” of the separation in the form of densely packed, often chemically functionalized beads. Once introduced to the column, it is the sample’s differential affinity for the solvent (“mobile”) and stationary phases that enables separation to occur, generally with sharper resolution than standard liquid chromatography. High performance liquid chromatographs can be used for analytical or preparative applications depending on the HPLC pump and the column size. HPLC columns are available in a variety of formats, including reverse phase, ion exchange, and size exclusion, which separate based on polarity, charge, and size, respectively.

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CompanyAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent Technologies
Item1290 Infinity III Bio Online LC System1260 Infinity III Analytical-Scale LC Purification System1260 Infinity III Method Development System1260 Infinity III Prime Online LC System1260 Infinity III SFC/UHPLC Hybrid System
Price
Flow Rateup to 5 mL/min0.001-10 mL/min0.001-10 mL/min0.001-5 mL/minUp to 5 mL/min
Max. Pressure1300 bar600 bar600 bar800 bar600 bar (SFC) / 800 bar (UHPLC)
DescriptionBiocompatible online UHPLC system for real-time bioprocess monitoring with up to 1300 bar.Analytical-scale HPLC purification system for preparative work up to 600 bar.Automated HPLC method development system with column and solvent switching.Online process analysis HPLC system for automated monitoring with real-time data. Quaternary pump, up to 800 bar.Hybrid SFC/UHPLC system with automated mode switching. 600 bar SFC, 800 bar UHPLC.
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