HPLC Instrument

High-performance liquid chromatography (HPLC) (also called high-pressure liquid chromatography) is a solution-phase technique for fractionation of complex samples. Unlike standard column chromatography, HPLC is an active process in which materials are pumped at high pressure through the separation column, which contains the so-called “stationary phase” of the separation in the form of densely packed, often chemically functionalized beads. Once introduced to the column, it is the sample’s differential affinity for the solvent (“mobile”) and stationary phases that enables separation to occur, generally with sharper resolution than standard liquid chromatography. High performance liquid chromatographs can be used for analytical or preparative applications depending on the HPLC pump and the column size. HPLC columns are available in a variety of formats, including reverse phase, ion exchange, and size exclusion, which separate based on polarity, charge, and size, respectively.

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CompanyAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent Technologies
Item1290 Infinity III Bio Online LC System1260 Infinity III Prime Analytical-Scale LC Purification System1290 Infinity III Bio Analytical-Scale LC Purification System1290 Infinity III HDR-DAD Impurity Analyzer System1290 Infinity III Online SPE System
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Detector(s)InquireInquireInquireHDR-DADInquire
Flow Rateup to 5 mL/min0.001-5 mL/minup to 5 mL/minup to 5 mL/min0.001-5 mL/min
Max. Pressure1300 bar800 bar1300 barup to 1300 bar1300 bar
DescriptionBiocompatible online UHPLC system for real-time bioprocess monitoring with up to 1300 bar.Flexible preparative HPLC/UHPLC system for analytical-scale purification, up to 800 bar.Biocompatible UHPLC purification system for multimilligram quantities, up to 1300 bar.Impurity analyzer with 30x higher dynamic range and UV sensitivity. UHPLC resolution at up to 1300 bar.Automated online solid phase extraction UHPLC system for analyte enrichment and matrix removal.
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