HPLC Instrument

High-performance liquid chromatography (HPLC) (also called high-pressure liquid chromatography) is a solution-phase technique for fractionation of complex samples. Unlike standard column chromatography, HPLC is an active process in which materials are pumped at high pressure through the separation column, which contains the so-called “stationary phase” of the separation in the form of densely packed, often chemically functionalized beads. Once introduced to the column, it is the sample’s differential affinity for the solvent (“mobile”) and stationary phases that enables separation to occur, generally with sharper resolution than standard liquid chromatography. High performance liquid chromatographs can be used for analytical or preparative applications depending on the HPLC pump and the column size. HPLC columns are available in a variety of formats, including reverse phase, ion exchange, and size exclusion, which separate based on polarity, charge, and size, respectively.

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CompanyAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent Technologies
Item1290 Infinity III 2D-LC System1260 Infinity III LC System1260 Infinity III Method Development System1260 Infinity III Prime Bio LC System1260 Infinity III Prime Online LC System
Price
Type2D-LC UHPLCModular HPLC/UHPLCInquireBio HPLC/UHPLCInquire
Detector(s)Multiple detector configurationsVWD, DAD, FLD, RID, ELSDInquireBio-Inert VWD, DAD, FLDInquire
Flow Rateup to 5 mL/min0.001-10 mL/min0.001-10 mL/min0.001-5 mL/min0.001-5 mL/min
Max. Pressure1300 bar600 bar (8700 psi)600 bar800 bar (11600 psi)800 bar
DescriptionTwo-dimensional LC UHPLC system for comprehensive separations and MS compatibility. Up to 1300 bar.Robust analytical HPLC system with binary, quaternary, or isocratic pump options, up to 600 bar. InfinityLab Assist technology.Automated HPLC method development system with column and solvent switching.Versatile bio HPLC/UHPLC system for bioseparations with biocompatible flow path and quaternary pump up to 800 bar.Online process analysis HPLC system for automated monitoring with real-time data. Quaternary pump, up to 800 bar.
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